Extração de DNA baseada em matriz para sequenciamento de próxima geração direcionado em amostras de escarro descontaminadas

1.9K vistas

06:07 min

6 de junho de 2025

1.9K vistas

Jennifer Williams^*¹ , Janré Steyn^*¹ , Emilyn Costa Conceição^*¹ , Felicia Bernita Wells¹ , Melanie Grobbelaar¹ , Nabila Ismail¹ , Yonas Ghebrekristos¹^,² , Christoffel Johannes Opperman¹^,²^,³^,⁴ , Sarishna Singh¹^,² , Jason Limberis⁵ , Fahd Naufal⁵ , Brendon Coenrad Mann¹ , Rebecca E. Colman⁶^,⁷ , Timothy Rodwell^*⁶^,⁷ , Robin Mark Warren^*¹ , John Z. Metcalfe^*⁵

¹Division of Molecular Biology and Human Genetics, SAMRC Centre for TB Research, DSI-NRF Centre of Excellence for Biomedical TB Research, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, ²National Health Laboratory Service, Green Point Tuberculosis Laboratory, ³Division of Medical Microbiology, Department of Pathology, University of Cape Town, ⁴Division of Medical Microbiology, Department of Pathology, University of Cape Town, ⁵Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, ⁶Department of Medicine, University of California, San Diego, School of Medicine, ⁷Foundation for Innovative New Diagnostics (FIND), Campus Biotech

^* These authors contributed equally

Apresentamos um método otimizado para extração de DNA de escarro descontaminado usando um método de extração baseado em matriz combinado com uma purificação de esferas magnéticas para sequenciamento de próxima geração direcionado a jusante de Mycobacterium tuberculosis.