Name: Novo modelo cirúrgico de roedores para estudar as opções de tratamento da dor do neuroma usando reinervação muscular direcionada através do nervo safeno (Vídeo) | JoVE Journal
Uploaded: 2025-11-14T13:55:00.000+00:00
Duration: 7 min
Description: Aprenda um modelo cirúrgico reprodutível em roedores para estudar dor de neuroma e avaliar tratamentos usando reinervação muscular direcionada pelo nervo safeno.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

playerTrialDesktop

<ol><li>Oliveira, K. M. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Time+course+of+traumatic+neuroma+development%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Time course of traumatic neuroma development.</a> PLoS One. 13 (7), e0200548(2018).</li>
<li>O'reilly, M. aR., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neuromas+as+the+cause+of+pain+in+the+residual+limbs+of+amputees.+An+ultrasound+study%5BTitle%5D)+AND+%22Clin+Radiol%22%5BJournal%5D)">Neuromas as the cause of pain in the residual limbs of amputees. An ultrasound study.</a> Clin Radiol. 71 (10), 1068.e1-1068.e6 (2016).</li>
<li>Anantavorasakul, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Surgery+for+lower+extremity+symptomatic+neuroma%3A+Long-term+outcomes%5BTitle%5D)+AND+%22J+Plast+Reconstr+Aesthet+Surg%22%5BJournal%5D)">Surgery for lower extremity symptomatic neuroma: Long-term outcomes.</a> J Plast Reconstr Aesthet Surg. 73 (8), 1456-1464 (2020).</li>
<li>Dieleman, J. P., Kerklaan, J., Huygen, F., Bouma, P. aD., Sturkenboom, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Incidence+rates+and+treatment+of+neuropathic+pain+conditions+in+the+general+population%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Incidence rates and treatment of neuropathic pain conditions in the general population.</a> Pain. 137 (3), 681-688 (2008).</li>
<li>List, E. B., Krijgh, D. D., Martin, E., Coert, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prevalence+of+residual+limb+pain+and+symptomatic+neuromas+after+lower+extremity+amputation%3A+A+systematic+review+and+meta-analysis%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Prevalence of residual limb pain and symptomatic neuromas after lower extremity amputation: A systematic review and meta-analysis.</a> Pain. 162 (7), 1906-1913 (2021).</li>
<li>Giladi, H., Scott, W., Shir, Y., Sullivan, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rates+and+correlates+of+unemployment+across+four+common+chronic+pain+diagnostic+categories%5BTitle%5D)+AND+%22J+Occup+Rehabil%22%5BJournal%5D)">Rates and correlates of unemployment across four common chronic pain diagnostic categories.</a> J Occup Rehabil. 25 (3), 648-657 (2015).</li>
<li>Ciaramitaro, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traumatic+peripheral+nerve+injuries%3A+Epidemiological+findings%2C+neuropathic+pain+and+quality+of+life+in+158+patients%5BTitle%5D)+AND+%22J+Peripher+Nerv+Syst%22%5BJournal%5D)">Traumatic peripheral nerve injuries: Epidemiological findings, neuropathic pain and quality of life in 158 patients.</a> J Peripher Nerv Syst. 15 (2), 120-127 (2010).</li>
<li>Moisset, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacological+and+non-pharmacological+treatments+for+neuropathic+pain%3A+Systematic+review+and+French+recommendations%5BTitle%5D)+AND+%22Rev+Neurol+%28Paris%29%22%5BJournal%5D)">Pharmacological and non-pharmacological treatments for neuropathic pain: Systematic review and French recommendations.</a> Rev Neurol (Paris). 176 (5), 325-352 (2020).</li>
<li>Hwang, C. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biology+and+pathophysiology+of+symptomatic+neuromas%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Biology and pathophysiology of symptomatic neuromas.</a> Pain. 165 (3), 550-564 (2024).</li>
<li>Gottlieb, R. W., Westenberg, R. F., Chen, N. C., Coert, J. H., Eberlin, K. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Long-term+outcomes+after+surgical+treatment+of+radial+sensory+nerve+neuromas%3A+Patient-reported+outcomes+and+rate+of+secondary+surgery%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Long-term outcomes after surgical treatment of radial sensory nerve neuromas: Patient-reported outcomes and rate of secondary surgery.</a> Plast Reconstr Surg. 147 (1), 101-111 (2021).</li>
<li>Langeveld, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Surgical+treatment+of+peripheral+nerve+neuromas%3A+A+systematic+review+and+meta-analysis%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Surgical treatment of peripheral nerve neuromas: A systematic review and meta-analysis.</a> Plast Reconstr Surg. 150 (4), 823e-834e (2022).</li>
<li>Baron, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Peripheral+neuropathic+pain%3A+From+mechanisms+to+symptoms%5BTitle%5D)+AND+%22Clin+J+Pain%22%5BJournal%5D)">Peripheral neuropathic pain: From mechanisms to symptoms.</a> Clin J Pain. 16 (2 Suppl), S12-S20 (2000).</li>
<li>Alant, J. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traumatic+neuroma+in+continuity+injury+model+in+rodents%5BTitle%5D)+AND+%22J+Neurotrauma%22%5BJournal%5D)">Traumatic neuroma in continuity injury model in rodents.</a> J Neurotrauma. 29 (8), 1691-1703 (2012).</li>
<li>Shamoun, F., Shamoun, V., Akhavan, A., Tuffaha, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Target+receptors+of+regenerating+nerves%3A+Neuroma+formation+and+current+treatment+options%5BTitle%5D)+AND+%22Front+Mol+Neurosci%22%5BJournal%5D)">Target receptors of regenerating nerves: Neuroma formation and current treatment options.</a> Front Mol Neurosci. 15, 859221(2022).</li>
<li>Cavadas, P. C., Tellez Palacios, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Treatment+of+neuroma+of+the+dorsal+branch+of+the+ulnar+nerve+with+transfer+to+the+distal+anterior+interosseous+nerve%5BTitle%5D)+AND+%22J+Hand+Surg+Glob+Online%22%5BJournal%5D)">Treatment of neuroma of the dorsal branch of the ulnar nerve with transfer to the distal anterior interosseous nerve.</a> J Hand Surg Glob Online. 2 (6), 363-364 (2020).</li>
<li>Dorsi, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+tibial+neuroma+transposition+%28tnt%29+model+of+neuroma+pain+and+hyperalgesia%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">The tibial neuroma transposition (tnt) model of neuroma pain and hyperalgesia.</a> Pain. 134 (3), 320-334 (2008).</li>
<li>Bennett, G. J., Xie, Y. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+peripheral+mononeuropathy+in+rat+that+produces+disorders+of+pain+sensation+like+those+seen+in+man%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man.</a> Pain. 33 (1), 87-107 (1988).</li>
<li>Castelnuovo, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Psychological+treatments+and+psychotherapies+in+the+neurorehabilitation+of+pain%3A+Evidences+and+recommendations+from+the+italian+consensus+conference+on+pain+in+neurorehabilitation%5BTitle%5D)+AND+%22Front+Psychol%22%5BJournal%5D)">Psychological treatments and psychotherapies in the neurorehabilitation of pain: Evidences and recommendations from the italian consensus conference on pain in neurorehabilitation.</a> Front Psychol. 7, 115(2016).</li>
<li>Decosterd, I., Woolf, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spared+nerve+injury%3A+An+animal+model+of+persistent+peripheral+neuropathic+pain%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Spared nerve injury: An animal model of persistent peripheral neuropathic pain.</a> Pain. 87 (2), 149-158 (2000).</li>
<li>Brakkee, E. M., Devinney, E., Eijkelkamp, N., Coert, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Surgery+and+behavioral+testing+in+the+tibial+neuroma+transposition+model+in+rats%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Surgery and behavioral testing in the tibial neuroma transposition model in rats.</a> J Vis Exp. (191), e64659(2023).</li>
<li>Kambiz, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Innervation+mapping+of+the+hind+paw+of+the+rat+using+evans+blue+extravasation%2C+optical+surface+mapping+and+casam%5BTitle%5D)+AND+%22J+Neurosci+Methods%22%5BJournal%5D)">Innervation mapping of the hind paw of the rat using evans blue extravasation, optical surface mapping and casam.</a> J Neurosci Methods. 229, 15-27 (2014).</li>
<li>Inquimbert, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Peripheral+nerve+injury+produces+a+sustained+shift+in+the+balance+between+glutamate+release+and+uptake+in+the+dorsal+horn+of+the+spinal+cord%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Peripheral nerve injury produces a sustained shift in the balance between glutamate release and uptake in the dorsal horn of the spinal cord.</a> Pain. 153 (12), 2422-2431 (2012).</li>
<li>Krijgh, D. D., List, E. B., Teunis, T., Bleys, R., Coert, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+origin+and+course+of+the+infrapatellar+branch+of+the+saphenous+nerve%3A+An+anatomical+study%5BTitle%5D)+AND+%22JPRAS+Open%22%5BJournal%5D)">The origin and course of the infrapatellar branch of the saphenous nerve: An anatomical study.</a> JPRAS Open. 34, 144-151 (2022).</li>
<li>Besmens, I. S., Brackertz, S., Nietlispach, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+cohort+study+on+neuropathic+pain+of+the+saphenous+nerve%E2%80%94factors+influencing+surgical+outcome%5BTitle%5D)+AND+%22Eur+J+Plast+Surg%22%5BJournal%5D)">A cohort study on neuropathic pain of the saphenous nerve—factors influencing surgical outcome.</a> Eur J Plast Surg. 46, 397-402 (2023).</li>
<li>Scura, P. V. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aScura%2C+PV+%22Disability+evaluation%22">Disability evaluation</a>. , StatPearls [internet]. Treasure Island, FL. (2023).</li>
<li>Chaplan, S. R., Bach, F. W., Pogrel, J. W., Chung, J. M., Yaksh, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+assessment+of+tactile+allodynia+in+the+rat+paw%5BTitle%5D)+AND+%22J+Neurosci+Methods%22%5BJournal%5D)">Quantitative assessment of tactile allodynia in the rat paw.</a> J Neurosci Methods. 53 (1), 55-63 (1994).</li>
<li>Scheuren, P. S., Rosner, J., Curt, A., Hubli, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pain-autonomic+interaction%3A+A+surrogate+marker+of+central+sensitization%5BTitle%5D)+AND+%22Eur+J+Pain%22%5BJournal%5D)">Pain-autonomic interaction: A surrogate marker of central sensitization.</a> Eur J Pain. 24 (10), 2015-2026 (2020).</li>
<li>Woolf, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Central+sensitization%3A+Implications+for+the+diagnosis+and+treatment+of+pain%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Central sensitization: Implications for the diagnosis and treatment of pain.</a> Pain. 152 (3 Suppl), S2-S15 (2011).</li>
<li>Latremoliere, A., Woolf, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Central+sensitization%3A+A+generator+of+pain+hypersensitivity+by+central+neural+plasticity%5BTitle%5D)+AND+%22J+Pain%22%5BJournal%5D)">Central sensitization: A generator of pain hypersensitivity by central neural plasticity.</a> J Pain. 10 (9), 895-926 (2009).</li>
<li>Roth, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+muscle+reinnervation+prevents+and+reverses+rat+pain+behaviors+after+nerve+transection%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Targeted muscle reinnervation prevents and reverses rat pain behaviors after nerve transection.</a> Pain. 164 (2), 316-324 (2023).</li>
<li>Dixon, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Efficient+analysis+of+experimental+observations%5BTitle%5D)+AND+%22Annu+Rev+Pharmacol+Toxicol%22%5BJournal%5D)">Efficient analysis of experimental observations.</a> Annu Rev Pharmacol Toxicol. 20, 441-462 (1980).</li>
<li>Wei, H., Viisanen, H., Amorim, D., Koivisto, A., Pertovaara, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dissociated+modulation+of+conditioned+place-preference+and+mechanical+hypersensitivity+by+a+trpa1+channel+antagonist+in+peripheral+neuropathy%5BTitle%5D)+AND+%22Pharmacol+Biochem+Behav%22%5BJournal%5D)">Dissociated modulation of conditioned place-preference and mechanical hypersensitivity by a trpa1 channel antagonist in peripheral neuropathy.</a> Pharmacol Biochem Behav. 104, 90-96 (2013).</li>
<li>Xie, J. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Activation+of+mesocorticolimbic+reward+circuits+for+assessment+of+relief+of+ongoing+pain%3A+A+potential+biomarker+of+efficacy%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Activation of mesocorticolimbic reward circuits for assessment of relief of ongoing pain: A potential biomarker of efficacy.</a> Pain. 155 (8), 1659-1666 (2014).</li>
<li>Dalm, B. D., Reddy, C. G., Howard, M. A., Kang, S., Brennan, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Conditioned+place+preference+and+spontaneous+dorsal+horn+neuron+activity+in+chronic+constriction+injury+model+in+rats%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Conditioned place preference and spontaneous dorsal horn neuron activity in chronic constriction injury model in rats.</a> Pain. 156 (12), 2562-2571 (2015).</li>
<li>Souza, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+muscle+reinnervation%3A+A+novel+approach+to+postamputation+neuroma+pain%5BTitle%5D)+AND+%22Clin+Orthop+Relat+Res%22%5BJournal%5D)">Targeted muscle reinnervation: A novel approach to postamputation neuroma pain.</a> Clin Orthop Relat Res. 472 (10), 2984-2990 (2014).</li>
<li>Asaoka, Y., Kato, T., Ide, S., Amano, T., Minami, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pregabalin+induces+conditioned+place+preference+in+the+rat+during+the+early%2C+but+not+late%2C+stage+of+neuropathic+pain%5BTitle%5D)+AND+%22Neurosci+Lett%22%5BJournal%5D)">Pregabalin induces conditioned place preference in the rat during the early, but not late, stage of neuropathic pain.</a> Neurosci Lett. 668, 133-137 (2018).</li>
<li>Sufka, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Conditioned+place+preference+paradigm%3A+A+novel+approach+for+analgesic+drug+assessment+against+chronic+pain%5BTitle%5D)+AND+%22Pain%22%5BJournal%5D)">Conditioned place preference paradigm: A novel approach for analgesic drug assessment against chronic pain.</a> Pain. 58 (3), 355-366 (1994).</li>
<li>Mckendrick, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ketamine+blocks+morphine-induced+conditioned+place+preference+and+anxiety-like+behaviors+in+mice%5BTitle%5D)+AND+%22Front+Behav+Neurosci%22%5BJournal%5D)">Ketamine blocks morphine-induced conditioned place preference and anxiety-like behaviors in mice.</a> Front Behav Neurosci. 14, 75(2020).</li>
<li>Deuis, J. R., Dvorakova, L. S., Vetter, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Methods+used+to+evaluate+pain+behaviors+in+rodents%5BTitle%5D)+AND+%22Front+Mol+Neurosci%22%5BJournal%5D)">Methods used to evaluate pain behaviors in rodents.</a> Front Mol Neurosci. 10, 284(2017).</li>
<li>Sotocinal, S. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+rat+grimace+scale%3A+A+partially+automated+method+for+quantifying+pain+in+the+laboratory+rat+via+facial+expressions%5BTitle%5D)+AND+%22Mol+Pain%22%5BJournal%5D)">The rat grimace scale: A partially automated method for quantifying pain in the laboratory rat via facial expressions.</a> Mol Pain. 7, 55(2011).</li>
<li>Draxler, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spontaneous%2C+voluntary%2C+and+affective+behaviours+in+rat+models+of+pathological+pain%5BTitle%5D)+AND+%22Front+Pain+Res+%28Lausanne%29%22%5BJournal%5D)">Spontaneous, voluntary, and affective behaviours in rat models of pathological pain.</a> Front Pain Res (Lausanne). 2, 672711(2021).</li>
<li>Panoutsopoulos, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Organoids%2C+assembloids%2C+and+novel+biotechnology%3A+Steps+forward+in+developmental+and+disease-related+neuroscience%5BTitle%5D)+AND+%22Neuroscientist%22%5BJournal%5D)">Organoids, assembloids, and novel biotechnology: Steps forward in developmental and disease-related neuroscience.</a> Neuroscientist. 27 (5), 463-472 (2021).</li>
</ol>

<table><tbody><tr><td>Carprofeno</td><td>Farmácia Veterinária Local</td><td>N/A</td><td>A farmácia veterinária local prepara a diluição do carprofeno</td></tr><tr><td>Cotonetes</td><td>Nobamed</td><td>974255</td><td>&mdash;</td></tr><tr><td>Barbeador elétrico</td><td>Esculapa</td><td>GT421</td><td></td></tr><tr><td>Eletrocauterização</td><td>Ferramentas Científicas Finas</td><td>18010-00</td><td>&mdash;</td></tr><tr><td>Etanol 70%</td><td>Interchema BV</td><td>400406</td><td>&mdash;</td></tr><tr><td>Ethilon 10.0</td><td>Johnson & Johnson</td><td>BV130-3: código 2820G</td><td></td></tr><tr><td>Ethilon 11.0</td><td>Johnson & Johnson</td><td>Código BV50-3L 2881G</td><td></td></tr><tr><td>Ethilon 4.0</td><td>Johnson & Johnson</td><td>1854G: código 1854G</td><td>-</td></tr><tr><td>Ethilon 6.0</td><td>Johnson & Johnson</td><td>BV130-5: código 2809G</td><td></td></tr><tr><td>Ethilon 8.0</td><td>Johnson & Johnson</td><td>BV130-5: código 2808G</td><td>&mdash;</td></tr><tr><td>Ethilon 9.0</td><td>Johnson & Johnson</td><td>BV130-3: código 2819G</td><td></td></tr><tr><td>Luvas de exame nitrilo</td><td>Sempermed GmbH</td><td>1065920</td><td></td></tr><tr><td>Proteção ocular</td><td>3M&trade; Saúde</td><td>71512-00001M</td><td></td></tr><tr><td>Esterilizador de Contas de Vidro</td><td>Equipamentos de laboratório VanPel</td><td>GBS300H</td><td></td></tr><tr><td>GraphPad Prisma</td><td>GraphPad Software, San Diego, Califórnia, EUA</td><td>&nbsp; Versão 10</td><td></td></tr><tr><td>Bolsa térmica</td><td>Ciência da Conduta</td><td>CS-HP-2005</td><td></td></tr><tr><td>Isoflo (isoflurano)</td><td>Zoetis / Dechra Produtos Veterinários</td><td>B506</td><td>&mdash;</td></tr><tr><td>Lidocaína 1%</td><td>Henry Schein</td><td>809755</td><td></td></tr><tr><td>Jaulas de fundo de malha</td><td>Stoelting Co.</td><td>57816, 57824</td><td>&mdash;</td></tr><tr><td>Micro pinças</td><td>Ferramentas Científicas Finas</td><td>11251-35</td><td>&mdash;</td></tr><tr><td>Suporte micro para agulhas</td><td>Ferramentas Científicas Finas</td><td>12076-12</td><td>&mdash;</td></tr><tr><td>Micro tesouras</td><td>Ferramentas Científicas Finas</td><td>15019-10</td><td>&mdash;</td></tr><tr><td>Pinça micro</td><td>Ferramentas Científicas Finas</td><td>11254-20</td><td>&mdash;</td></tr><tr><td>NaCl 0,9%</td><td>Trademed</td><td>H7 1000-FRE</td><td>&mdash;</td></tr><tr><td>Agulha 25G e horários; 1"</td><td>Becton, Dickinson & Empresa</td><td>300400</td><td></td></tr><tr><td>Suporte de agulha</td><td>Ferramentas Científicas Finas</td><td>12004-16</td><td>&mdash;</td></tr><tr><td>Estimulador nervoso</td><td>&nbsp; (VARI-STIM Localizador de nervos)</td><td>REF 856200</td><td></td></tr><tr><td>Software RandoMice</td><td>van Eineige et al.&nbsp; Leiden</td><td>Versão 1.1.7</td><td></td></tr><tr><td>Sonda retal de roedor</td><td>Instrumentos de Precisão Mundial</td><td>NC9506514</td><td></td></tr><tr><td>Bisturi</td><td>Ferramentas Científicas Finas</td><td>10003-12</td><td>&mdash;</td></tr><tr><td>Tesoura</td><td>Ferramentas Científicas Finas</td><td>14001-12</td><td>&mdash;</td></tr><tr><td>Avaliador sensorial</td><td>Instrumentação Linton</td><td>514007&ndash; 514015</td><td>Monofilamentos de 0,6 g a 15 g</td></tr><tr><td>Microscópio cirúrgico estereoscópico</td><td>Leica</td><td>A60 F</td><td>&mdash;</td></tr><tr><td>Compressas estéreis de gaze</td><td>Nobamed</td><td>740113</td><td></td></tr><tr><td>Pomada lubrificante ocular estéril</td><td>Alcon&nbsp;</td><td>&lrm; B00BRLSC8S</td><td></td></tr><tr><td>Folha estéril com furo</td><td>Evercare / OneMed</td><td>1555-01</td><td>&mdash;</td></tr><tr><td>Lâmina cirúrgica nº 15</td><td>Ferramentas Científicas Finas</td><td>10015-00</td><td>&mdash;</td></tr><tr><td>Máscara cirúrgica</td><td>Medline Industries, Inc.</td><td>MSK1002</td><td></td></tr><tr><td>Seringa Luer Lock 2 ml</td><td>Becton, Dickinson & Empresa</td><td>300613</td><td></td></tr><tr><td>Pinça</td><td>Ferramentas Científicas Finas</td><td>11617-12</td><td>&mdash;</td></tr></tbody></table>

novo modelo cirúrgico de roedores para estudar as opções de tratamento da dor do neuroma usando reinervação muscular direcionada através do nervo safeno

Novel Surgical Rodent Model for Studying Neuroma Pain Treatment Options using Targeted Muscle Reinnervation Through the Saphenous Nerve

Novo modelo cirúrgico de roedores para estudar as opções de tratamento da dor do neuroma usando reinervação muscular direcionada através do nervo safeno

This protocol describes a novel surgical model to study nerve pain and surgical treatment in rats. Traditional neuroma models use mixed nerves, although ...

novel-surgical-rodent-model-for-studying-neuroma-pain-treatment

Research

JoVE Journal

Neuroscience

Neurociência

Poupado do Nervo modelo animal de lesão é descrita aqui como um modelo do rato da dor neuropática periférica seguintes denervação parcial do nervo ciático por a lesão do tibial e nervo peroneal comum ramos, deixando o nervo sural restante intacto. Modificação comportamental resultante da alodinia mecânica é quantificada por filamentos de von Frey.

Poupado da lesão do nervo Model (SNI) de alodinia mecânica induzida em camundongos

Fonte: Brakkee, E. M., et al. Cirurgia e Teste Comportamental no Modelo de Transposição do Neuroma Tibial em Ratos. J. Vis. Exp. (2023). Este vídeo demonstra um procedimento para gerar um modelo de transposição de neuroma tibial de rato (TNT). Depois de expor o nervo tibial na perna traseira de um rato anestesiado, faça o transeccionamento do nervo. Em seguida, crie um túnel subcutâneo, passe o nervo cortado pelo túnel e suture-o no lugar. Na ausência de um alvo para reinervação, as...

Gerando um modelo de rato de transposição de neuroma tibial

A dor neuropática é uma conseqüência de uma lesão ou doença que afeta o sistema somatossensorial. O "modelo cuff" da dor neuropática em ratos consiste na implantação de um manguito de polietileno em todo o ramo principal do nervo ciático. A alodinia mecânica é testado usando filamentos de von Frey.

O ciático Nervo cuffing Modelo de Dor Neuropática em Ratos

A cirurgia modificada é um método simplificado para o modelo de lesão nervosa poupada de camundongo ou rato que requer apenas uma ligadura e um corte para lesionar os nervos fibular e sural comuns.

Modelo de cirurgia de lesão nervosa poupada modificada de dor neuropática em camundongos

A ligadura parcial do nervo ciático induz dor neuropática crônica de longa duração, caracterizada por respostas exageradas a estímulos térmicos e mecânicos. Este modelo murino de dor neuropática é comumente usado para estudar terapias inovadoras para o tratamento da dor. Este artigo descreve em detalhes o procedimento cirúrgico para melhorar a padronização e reprodutibilidade.

Ligadura parcial do nervo ciático: um modelo murino de dor neuropática crônica para estudar o efeito antinociceptivo de novas terapias

Este protocolo descreve o modelo de transposição do neuroma tibial, que envolve uma lesão do nervo tibial com subsequente transposição da extremidade proximal do nervo para uma posição subcutânea pré-tibial ou lateral. O teste comportamental da dor do neuroma e da hiperalgesia plantar é quantificado usando monofilamentos de Von Frey.

Cirurgia e Testes Comportamentais no Modelo de Transposição do Neuroma Tibial em Ratos

Aqui, descrevemos o procedimento cirúrgico para realizar a cirurgia de Interface Regenerativa de Nervos Periféricos (RPNI) para o tratamento da dor neuropática pós-amputação no contexto de um estudo controlado randomizado (RCT) internacional (ClinicalTrials.gov, NCT05009394). O ECR compara o RPNI com duas outras técnicas cirúrgicas, a saber, Reinervação Muscular Direcionada (TMR) e excisão de neuroma combinada com transposição intramuscular.

Interface Regenerativa de Nervos Periféricos: Protocolo Cirúrgico para um Estudo Controlado Randomizado em Dor Pós-amputação

O protocolo descreve o procedimento cirúrgico para o tratamento da dor pós-amputação usando a reinervação muscular direcionada (TMR). A TMR será comparada com duas outras técnicas cirúrgicas, especificamente a Interface Regenerativa do Nervo Periférico (RPNI) e a excisão do neuroma, seguida de enterramento imediato no músculo no contexto de um estudo internacional randomizado controlado.

Reinervação Muscular Direcionada: Protocolo Cirúrgico para um Estudo Controlado Randomizado em Dor Pós-amputação

Este protocolo demonstra como realizar uma amputação abaixo do joelho em ratos e realizar imediatamente a inervação muscular direcionada (iTMR) para investigar estratégias de tratamento e prevenção da dor relacionada à amputação e para melhor compreender os mecanismos da dor associados à amputação.

Modelo de amputação do membro posterior do rato abaixo do joelho e reinervação muscular imediata direcionada

Usando métodos de ressonância magnética funcionais e comportamentais para determinar a representação neural do valor subjetivo de opções de risco e ambígua no cérebro humano.

Medir o valor subjetivo de Opções de risco e ambígua usando Economia Experimental e funcional métodos de ressonância magnética

Aprenda um modelo cirúrgico reprodutível em roedores para estudar dor de neuroma e avaliar tratamentos usando reinervação muscular direcionada pelo nervo safeno.

Novo modelo cirúrgico de roedores para estudar as opções de tratamento da dor do neuroma usando reinervação muscular direcionada através do nervo safeno (Vídeo) | JoVE Journal

Novo modelo cirúrgico de roedores para estudar as opções de tratamento da dor do neuroma usando reinervação muscular direcionada através do nervo safeno

Explorar mais vídeos