Name: Determinação da Biomassa Microbiana no Solo usando PCR Digital por Gotículas (ddPCR) (Vídeo) | JoVE Journal
Uploaded: 2026-04-24T14:20:00.000+00:00
Duration: 7 min 46 s
Description: Aprenda a quantificar biomassa microbiana em amostras de solo usando ddPCR com genes 16S e 18S rRNA. Este método permite quantificação absoluta de bactérias e fungos para avaliação da saúde do solo.

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</ol>

<table><tbody><tr><td>Bettersizer S3 Plus&nbsp;</td><td>Bettersize, China</td><td>BT-803</td><td></td></tr><tr><td>Centrífuga</td><td>Termociência &nbsp;</td><td>SL16&nbsp;</td><td></td></tr><tr><td>Clorofórmio &nbsp;</td><td>ThermoScientific</td><td>L14759. AU&nbsp;</td><td></td></tr><tr><td>Placas de 96 Poços ddPCR</td><td>Bio-Rad, Watford, Reino Unido</td><td>12001925</td><td></td></tr><tr><td>ddPCR Óleo Leitor de Gotas&nbsp;</td><td>Bio-Rad, Watford, Reino Unido</td><td>1863004</td><td></td></tr><tr><td>Dessecador&nbsp;</td><td>Davisil</td><td></td><td></td></tr><tr><td>Suporte de Cartucho DG8</td><td>Bio-Rad, Watford, Reino Unido</td><td>1863051</td><td></td></tr><tr><td>Cartuchos DG8 para Gerador de Gotículas QX200/QX100</td><td>Bio-Rad, Watford, Reino Unido</td><td>1864008</td><td></td></tr><tr><td>Juntas DG8 para Gerador de Gotículas QX200/QX100</td><td>Bio-Rad, Watford, Reino Unido</td><td>P33265</td><td></td></tr><tr><td>Kit pro DNeasy Power Soil & nbsp;</td><td>Qiagen, Alemanha</td><td>47017</td><td></td></tr><tr><td>Kit de ensaio de larga faixa (dsDNA) de larga distância (BR)</td><td>Invitrogen, Alemanha</td><td>P33230</td><td></td></tr><tr><td>FastPrep-24 5G&nbsp;</td><td>MP Biomédicas, Reino Unido</td><td>116005500</td><td></td></tr><tr><td>GridION&nbsp;</td><td>Oxford Nanopore Technologies, Reino Unido</td><td>GRD-MK1CAPX</td><td></td></tr><tr><td>K2SO4</td><td>Merck, Reino Unido</td><td>7778-80-5</td><td></td></tr><tr><td>Esferas magnéticas Mag-Bind</td><td>Omega Bio-tek, EUA</td><td>M1378-01</td><td></td></tr><tr><td>Célula de fluxo MinION</td><td>Oxford Nanopore Technologies, Reino Unido</td><td>FLO-MIN114</td><td></td></tr><tr><td>Forno abafado</td><td>Carbolite & nbsp;</td><td>AAF1100</td><td></td></tr><tr><td>Kit nativo de código de barras 24</td><td>Oxford Nanopore Technologies, Reino Unido</td><td>SQK-NBD114.24</td><td></td></tr><tr><td>Vedação térmica da placa PCR, folha de alumínio, perfurável &nbsp;</td><td>Bio-Rad, Watford, Reino Unido</td><td>1814040</td><td></td></tr><tr><td>Selante de placas PCR PX1</td><td>Bio-Rad, Watford, Reino Unido</td><td>1814000</td><td></td></tr><tr><td>Fluorômetro de Qubits</td><td>Invitrogen, Alemanha</td><td>Q33226</td><td></td></tr><tr><td>Sistema Digital de PCR com Gotejos QX200</td><td>Bio-Rad, Watford, Reino Unido</td><td>1864001</td><td></td></tr><tr><td>QX200 ddPCR EvaGreen Supermix</td><td>Bio-Rad, Watford, Reino Unido</td><td>186-4033</td><td></td></tr><tr><td>Óleo de geração de gotículas QX200 para EvaGreen & nbsp;</td><td>Bio-Rad, Watford, Reino Unido</td><td>1864005</td><td></td></tr><tr><td>QXDx Gerador de Gotas&nbsp;</td><td>Bio-Rad, Watford, Reino Unido</td><td>12001049</td><td></td></tr><tr><td>SENSOQUEST LABCYCLER&nbsp;</td><td>Geneflow, Alemanha</td><td>1120280125</td><td></td></tr></tbody></table>

determinação da biomassa microbiana no solo usando pcr digital por gotículas (ddpcr)

Determination of Microbial Biomass in Soil using Digital Droplet PCR (ddPCR)

Determinação da Biomassa Microbiana no Solo usando PCR Digital por Gotículas (ddPCR)

Soil represents the largest terrestrial pool of organic carbon, yet more than 133 Gt of carbon has been lost from soils over the past two centuries. ...

determination-microbial-biomass-soil-using-digital-droplet-pcr

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JoVE Journal

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Um ensaio de PCR Duplex Digital para a simultânea Quantificação da<em&gt; Enterococcus spp.</em&gt; Ea Human Fecal-associado HF183 marcador no Waters

Um método sensível e preciso para quantificação de microRNAs livres de células usando uma química baseada em corante e tecnologia de PCR digital de gotículas é descrito.

Circulando MicroRNA Quantificação Usando DNA de ligação Dye Química e Gota Digital PCR

Detecção rápida e exata de patógenos no local, especialmente de solo-carregadas patógenos vegetais, é crucial para prevenir mais produção de inóculo e proliferação de doenças de plantas no campo. O método desenvolvido aqui, usando um sistema de detecção de PCR em tempo real portátil permite o diagnóstico no local em condições de campo.

Deteção Molecular no local de Phytopathogens solo-carregadas usando um sistema portátil de PCR em tempo real

Aqui, descrevemos um protocolo para obter dados de sequência de amplicons da rizosfera, solo e raiz endosphere microbiomes. Esta informação pode ser usada para investigar a composição e diversidade de comunidades microbianas associadas a planta e é apropriada para o uso com uma ampla gama de espécies de plantas.

Explorando o microbioma da raiz: extraindo dados da comunidade bacteriana do solo

É descrito aqui um procedimento de funcionamento padrão simplificado para o perfilamento do microbioma usando o sequenciação metagenômica 16s rRNA e a análise usando ferramentas livremente disponíveis. Este protocolo ajudará os investigadores que são novos ao campo do microbioma assim como aqueles que exigem actualizações em métodos para conseguir o perfilamento bacteriano em uma definição mais elevada.

Análise de microbiota usando PCR de duas etapas e sequenciamento do gene 16S rRNA de próxima geração

Aqui apresentamos um protocolo para medir o número absoluto de cópias mitocondriais (mt)DNA e os níveis de heteroplasmia de deleção do mtDNA em células únicas.

Medindo o Número de Cópias de DNA Mitocondrial de Célula Única e a Heteroplasmia Usando a Reação em Cadeia da Polimerase de Gotículas Digitais

Aqui, apresentamos um protocolo para o desenvolvimento e validação de boas práticas laboratoriais na detecção aderente de vetores virais adenoassociados em lágrimas humanas por reação em cadeia da polimerase digital em gotículas em apoio ao desenvolvimento clínico de vetores de terapia gênica.

Um ensaio de reação em cadeia da polimerase digital validável em gotículas para a detecção de vetores virais adenoassociados em estudos de biosshedding de lágrimas

A quantificação precisa das cópias do genoma do vetor do vírus adeno-associado (AAV) é crítica, mas um protocolo padronizado ainda não foi estabelecido. Este protocolo descreve um método validado para preparar amostras de AAV purificadas e conduzir a reação em cadeia da polimerase de gotículas digitais (dd_PCR) para quantificar de forma confiável o título do genoma viral.

Quantificação de genomas virais adeno-associados em amostras de vetores purificados por reação em cadeia da polimerase por gotículas digitais

Formas confiáveis e precisas de análise de DNA implantável em campo são cruciais para muitas indústrias. Este artigo oferece dois métodos para análise microbiana ambiental em campo: 1) o isolamento de DNA microbiano de amostras ambientais usando um kit de extração biológica de campo, e 2) a análise do DNA via unidade qPCR de campo.

Análise de DNA microbiano em campo usando um kit de extração biológica de campo e uma unidade qPCR de campo

Aprenda a quantificar biomassa microbiana em amostras de solo usando ddPCR com genes 16S e 18S rRNA. Este método permite quantificação absoluta de bactérias e fungos para avaliação da saúde do solo.

Determinação da Biomassa Microbiana no Solo usando PCR Digital por Gotículas (ddPCR)

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