Name: Mapeamento da Imunidade Infantil com Entrada Mínima: Perfil Integrativo de Célula Única e Multiômico (Vídeo) | JoVE Journal
Uploaded: 2026-04-03T12:35:00.000+00:00
Duration: 10 min 29 s
Description: Aprenda a realizar perfilagem imune multidimensional em neonatos com volumes mínimos de sangue. Este protocolo integra citometria de fluxo, proteômica e sequenciamento de RNA de célula única para análise longitudinal do desenvolvimento imunológico infantil.

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</ol>

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mapeamento da imunidade infantil com entrada mínima: perfil integrativo de célula Única e multiômico

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling

Mapeamento da Imunidade Infantil com Entrada Mínima: Perfil Integrativo de Célula Única e Multiômico

At birth, the neonatal immune system is abruptly confronted with a radically different environment, rich in microbial and environmental stimuli. ...

mapping-infant-immunity-with-minimal-input-integrative-single-cell

Research

JoVE Journal

Immunology and Infection

Imunologia e Infecção

Este protocolo descreve a forma de avaliar a expressão de uma grande variedade de genes ao nível clonal. De uma única célula RT-qPCR produz resultados altamente confiáveis, com uma forte sensibilidade para centenas de amostras e genes.

Expressão Gênica de uma única célula Usando Multiplex RT-qPCR Caracterizar Heterogeneidade de rara populações linfóides

Aqui nós descrevemos uma abordagem proteômica de célula única para avaliar imunológico fenotípico e funcional (indução de citocinas intracelulares) alterações nas amostras de sangue periférico, analisadas através de citometria de massa.

Análise de célula única do imunofenótipo e produção de citocinas no sangue periférico através de citometria de massa

Granel gene expressão medições nuvem célula individual diferenças nas populações de células heterogêneas. Aqui, descrevemos um protocolo para análise de expressão do gene como unicelular e índice classificação por Florescence ativado celular classificação (FACS) pode ser combinado para delinear a heterogeneidade e immunophenotypically caracterizam populações de células molecularmente distinta.

Uma combinatória célula única abordagem para caracterizar o Molecular e imunofenotípica heterogeneidade de tronco humano e populações de Progenitor

A imunoprecipitação quantitativa Multiplex (QMI) utiliza citometria de fluxo para detecção sensível de diferenças na abundância de interações proteína-proteína direcionadas entre duas amostras. O QMI pode ser realizado usando uma pequena quantidade de biomaterial, não requer Tags geneticamente projetadas e pode ser adaptado para qualquer rede de interação protéica previamente definida.

Quantificação da dinâmica da rede de interação de proteínas usando co-imunoprecipitação multiplexada

O uso de uma abordagem baseada em RNA para determinar perfis imunológicos quantitativos de tecidos tumorais sólidos e aproveitar coortes clínicas para a descoberta de biomarcadores imuno-oncologia é descrito através de protocolos moleculares e informativos.

Modelagem imunológica preditiva de tumores sólidos

Aqui, descrevemos uma estratégia simples e acessível para visualizar, quantificar e mapear células imunes em seções de tecido tumoral parafina fixada em formalina. Essa metodologia combina técnicas de imagem e análise digital existentes com o objetivo de ampliar a capacidade de multiplexação e a análise multiparâmetro de ensaios de imagem.

Visualização, Quantificação e Mapeamento de Populações de Células Imunes no Microambiente tumoral

Na era da imunoterapia e do perfil genômico de célula única, a biologia do câncer requer novas ferramentas in vitro e computacionais para investigar a interface tumor-imune em um contexto espaço-temporal adequado. Descrevemos protocolos para explorar co-culturas microfluídicas imunes a tumores em cenários 2D e 3D, compatíveis com monitoramento dinâmico e multiparamétrico de funções celulares.

Modelos de Co-Cultura Microfluídica para Dissecar a Resposta Imune em Microambientes Tumorais in vitro

Este protocolo combina citometria de fluxo e sequenciamento de RNA de célula única para isolar e caracterizar os estados das células microgliais no cerebelo de cérebros de camundongos pós-natais precoces. Ele usa dissociação enzimática, centrifugação de Percoll e imunocoloração para revelar a heterogeneidade da microglia e melhorar a compreensão de seus papéis no desenvolvimento e doença cerebelar.

Citometria de fluxo e análise de célula única para caracterizar a ativação da micróglia no desenvolvimento cerebral de camundongos pós-natais precoces

Uma célula de ensaio de morte com base em plantas para PTI Nicotiana benthamiana é descrito.

Ensaio para Pathogen-Associated Padrão Molecular (PAMP) Triggered-Imunidade (PTI) em Plantas

Seleção de Plasmodium falciparum Parasitas para Cytoadhesion para células do cérebro humano endotelial

Aprenda a realizar perfilagem imune multidimensional em neonatos com volumes mínimos de sangue. Este protocolo integra citometria de fluxo, proteômica e sequenciamento de RNA de célula única para análise longitudinal do desenvolvimento imunológico infantil.

Mapeamento da Imunidade Infantil com Entrada Mínima: Perfil Integrativo de Célula Única e Multiômico

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