Name: Um modelo de lesão por hipóxia-reoxigenação em cardióides humanos auto-montantes (Vídeo) | JoVE Journal
Uploaded: 2026-03-17T12:35:00.000+00:00
Duration: 10 min 41 s
Description: Aprenda a modelar lesão por hipóxia-reoxigenação em cardióides humanos auto-montantes derivados de iPSCs. Este protocolo permite estudar respostas a lesões cardíacas e testar intervenções terapêuticas em contexto humano.

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</ol>

<table><tbody><tr><td>Solução de Accutase</td><td>Sigma-Aldrich</td><td>A6964-500ML</td><td></td></tr><tr><td>Anticorpo anti-cardíaco Troponina T</td><td>Abcam</td><td>EPR20266</td><td></td></tr><tr><td>Suplemento de B-27 (50X), sem soro</td><td>Gibco</td><td>17504044</td><td></td></tr><tr><td>Suplemento de B-27, menos insulina</td><td>Gibco</td><td>A1895602</td><td></td></tr><tr><td>Leitor de placas BioTek Synergy Neo2</td><td>BioTek</td><td></td><td></td></tr><tr><td>Cal-520, AM</td><td>AAT Bioquest</td><td>21131</td><td></td></tr><tr><td>CardioCount V1</td><td>Karra Lab</td><td>PMID: 38984052</td><td></td></tr><tr><td>Linha celular (Homo sapiens, masculino) - Linha iPSC DU11</td><td>Núcleo de Células-Tronco da Universidade Duke</td><td></td><td></td></tr><tr><td>Lâminas de microscópio carregadas, vidro branco</td><td>VWR</td><td>89500-496</td><td></td></tr><tr><td>CHIR 99021</td><td>Tocris</td><td>4423</td><td></td></tr><tr><td>CloneR</td><td>Tecnologias STEMCELL</td><td>5888</td><td></td></tr><tr><td>Microplacas de fixação Corning 96-Well Clear Ultra Low Fixation</td><td>Fisher</td><td>7007</td><td></td></tr><tr><td>Reservatórios de Pipetagem Descartáveis</td><td>VWR</td><td>89094-678</td><td></td></tr><tr><td>DMEM/F-12, HEPES</td><td>Gibco</td><td>11330057</td><td></td></tr><tr><td>Microscópio Fluorescente EVOS</td><td>EVOS</td><td></td><td></td></tr><tr><td>Matriz de Membrana Basal com Fator de Crescimento Reduzido Geltrex, livre de LDEV, qualificada para células-tronco</td><td>Gibco</td><td>A1413302</td><td></td></tr><tr><td>Activina Humana: Uma Proteína Recombinante</td><td>PEPROTECH INC/Life Technologies</td><td>120-14E-50UG</td><td></td></tr><tr><td>IWP-2</td><td>Tecnologias STEMCELL</td><td>72124</td><td></td></tr><tr><td>Ácido L-Ascórbico (Cristalino/ACS Certificado)</td><td>Fisher Scientific</td><td>A61-100</td><td></td></tr><tr><td>Anti-Vimentina para camundongos</td><td>DSHB</td><td>AMF-17b</td><td></td></tr><tr><td>mTeSR Plus</td><td>Tecnologias STEMCELL</td><td>100-0276</td><td></td></tr><tr><td>Placas de Cultura Celular Multipoço &ndash; 12 poço</td><td>VWR</td><td>10062-894</td><td></td></tr><tr><td>F-127 Plurônico</td><td>Sigma-Aldrich</td><td>P2443</td><td></td></tr><tr><td>ReLeSR</td><td>Tecnologias STEMCELL</td><td>100-0483</td><td></td></tr><tr><td>RPMI 1640 Médio</td><td>Gibco</td><td>11875119</td><td></td></tr><tr><td>RPMI 1640 Médio, sem fenólico vermelho</td><td>Gibco</td><td>11835-030</td><td></td></tr><tr><td>Tiazovivina</td><td>Tecnologias STEMCELL</td><td>72254</td><td></td></tr><tr><td>Composto Tecido-Tecido O.C.T.</td><td>SAKURA FINETEK USA INC (VWR)</td><td>25608-930</td><td></td></tr><tr><td>Microscópio Confocal Zeiss Spinning Disk</td><td>Zeiss</td><td></td><td></td></tr></tbody></table>

um modelo de lesão por hipóxia-reoxigenação em cardióides humanos auto-montantes

A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids

Um modelo de lesão por hipóxia-reoxigenação em cardióides humanos auto-montantes

Without rapid revascularization, ischemic heart disease leads to irreversible loss of cardiomyocytes, scar formation, and decreased cardiac function. The ...

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Medicine

Medicina

Este manuscrito descreve a criação de tecidos cardíacos projetados definidos usando expressão de marcadores de superfície e classificação celular. Os tecidos definidos podem então ser usados em um biorreator multitecido para investigar os mecanismos da terapia com células cardíacas, a fim de fornecer um sistema modelo funcional, mas controlado, do coração humano.

Construção de Engineered Definido cardíaca humana Tecidos para estudar os mecanismos da terapia celular cardíaca

Aqui, apresentamos um protocolo para descrever um modelo de recuperação simples extracorpórea sem transfusão ou inotrópicos em um rato. Este modelo permite o estudo de longo prazo várias sequelas de órgão de bypass cardiopulmonar.

Um modelo de recuperação de Bypass cardiopulmonar sem transfusão ou inotrópicos em ratos

Apresentamos um modelo de doença isquêmica do coração utilizando cardiomiócitos derivados de células-tronco pluripotentes induzidas pelo homem, juntamente com um método de avaliação quantitativa dos danos teciduais causados pela isquemia. Este modelo pode fornecer uma plataforma útil para triagem de medicamentos e mais pesquisas sobre doenças isquêmicas do coração.

Modelo de Doença Cardíaca Isquêmica e Comparação Baseada em Vídeo de Contração de Cardiomiócitos usando cardiomiócitos derivados do hiPSC

Os efeitos distintos de diferentes graus de hipotermia na proteção do miocárdio não foram minuciosamente avaliados. O objetivo do presente estudo foi quantificar os níveis de morte celular após diferentes tratamentos de hipotermia em um modelo baseado em cardiomiócito humano, estabelecendo as bases para futuras pesquisas moleculares aprofundadas.

Avaliação in vitro da proteção do miocárdio após hipotermia-pré-condicionamento em um modelo de miócitos cardíacos humanos

O objetivo deste protocolo é explicar e demonstrar o desenvolvimento de um modelo microfluido tridimensional (3D) de tecido cardíaco humano altamente alinhado, composto por cardiomiócitos derivados de células-tronco co-cultivados com fibroblastos cardíacos (CFs) dentro de um hidrogel biomimético, à base de colágeno, para aplicações em engenharia de tecido cardíaco, triagem de medicamentos e modelagem de doenças.

Desenvolvimento de tecido cardíaco humano organizado 3D dentro de uma plataforma microfluida

A distância espacial é um parâmetro fundamental na avaliação da lesão por hipóxia/reoxigenação em um modelo de cocultura de camadas separadas de células células endoteliais e cardiomiócitos, sugerindo, pela primeira vez, que a otimização do ambiente espacial de co-cultura é necessária para fornecer um modelo in vitro favorável para testar o papel das células endoteliais na proteção de cardiomiócitos.

Desenvolvimento de um modelo de co-cultura celular para imitar isquemia cardíaca/reperfusão in vitro

Aqui, descrevemos um protocolo para criar organoides cardíacos humanos relevantes de desenvolvimento (hHOs) usando eficientemente células-tronco pluripotentes humanas por auto-organização. O protocolo conta com a ativação sequencial de pistas de desenvolvimento e produz tecidos cardíacos humanos altamente complexos e funcionalmente relevantes.

Gerando organoides cardíacos humanos auto-montados derivados de células-tronco pluripotentes

Tecidos cardíacos tridimensionais bioprojetados usando cardiomiócitos derivados de células-tronco têm emergido como modelos promissores para estudar o miocárdio humano saudável e doente in vitro , enquanto recapitulam aspectos-chave do nicho cardíaco nativo. Este manuscrito descreve um protocolo para fabricação e análise de tecidos cardíacos projetados de alto conteúdo gerados a partir de cardiomiócitos derivados de células-tronco pluripotentes induzidos por humanos.

Projetando um Biorreator para Melhorar a Aquisição de Dados e Modelar o Rendimento de Tecidos Cardíacos Projetados

Aqui, descrevemos um protocolo in vitro para modelar danos por hipóxia/reoxigenação nas células epiteliais tubulares proximais, altamente metabólicas. Este modelo foi projetado para induzir danos induzidos por estresse metabólico, que podem ser medidos pela expressão de marcadores de dano dos túbulos proximais e avaliação da função respiratória mitocondrial.

Modelagem de Hipóxia/Lesão por Reoxigenação em Células Epiteliais Tubulares Proximais

Um modelo de contusão grave lesão da medula espinhal é descrito. As etapas detalhadas no pré-operatório, operatório e pós-operatório são descritos para obter um modelo consistente.

Um modelo de contusão grave de lesão medular em ratos

Aprenda a modelar lesão por hipóxia-reoxigenação em cardióides humanos auto-montantes derivados de iPSCs. Este protocolo permite estudar respostas a lesões cardíacas e testar intervenções terapêuticas em contexto humano.

Um modelo de lesão por hipóxia-reoxigenação em cardióides humanos auto-montantes

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