Name: Geração do modelo de camundongo de ativação imunológica materna gestacional precoce para avaliar a inflamação pré-natal no neurodesenvolvimento (Vídeo) | JoVE Journal
Uploaded: 2026-03-24T13:05:00.000+00:00
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Description: Aprenda a gerar um modelo de ativação imunológica materna em camundongos durante a gestação precoce para avaliar a inflamação pré-natal no neurodesenvolvimento. Este protocolo padronizado melhora a reprodutibilidade.

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<li>Atanasova, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immune+activation+during+pregnancy+exacerbates+ASD-related+alterations+in+Shank3-deficient+mice%5BTitle%5D)+AND+%22Mol.+Autism%22%5BJournal%5D)">Immune activation during pregnancy exacerbates ASD-related alterations in Shank3-deficient mice.</a> Mol. Autism. 14, 1(2023).</li>
<li>Abazyan, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prenatal+Interaction+of+Mutant+DISC1+and+Immune+Activation+Produces+Adult+Psychopathology%5BTitle%5D)+AND+%22Biol.+Psychiatry%22%5BJournal%5D)">Prenatal Interaction of Mutant DISC1 and Immune Activation Produces Adult Psychopathology.</a> Biol. Psychiatry. 68, 1172-1181 (2010).</li>
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<li>Guma, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Investigating+the+%26%2334%3Btwo-hit+hypothesis%26%2334%3B%3A+Effects+of+prenatal+maternal+immune+activation+and+adolescent+cannabis+use+on+neurodevelopment+in+mice%5BTitle%5D)+AND+%22Prog.+Neuropsychopharmacol.+Biol.+Psychiatry%22%5BJournal%5D)">Investigating the &#34;two-hit hypothesis&#34;: Effects of prenatal maternal immune activation and adolescent cannabis use on neurodevelopment in mice.</a> Prog. Neuropsychopharmacol. Biol. Psychiatry. 120, 110642(2023).</li>
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<li>Sal-Sarria, S., Conejo, N. M., González-Pardo, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Maternal+immune+activation+and+its+multifaceted+effects+on+learning+and+memory+in+rodent+offspring%3A+A+systematic+review%5BTitle%5D)+AND+%22Neurosci.+Biobehav.+Rev%22%5BJournal%5D)">Maternal immune activation and its multifaceted effects on learning and memory in rodent offspring: A systematic review.</a> Neurosci. Biobehav. Rev. 164, 105844(2024).</li>
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</ol>

<table><tbody><tr><td>Animal weighing scale</td><td>Kent Scientific</td><td>SCL-4000</td><td>Para pesagem de mcie</td></tr><tr><td>BD Lo-Dose U-100 Insulin syringes - 28G</td><td>Fisher Scientific</td><td>14-826-79</td><td>Para PIC, injeção intraperitoneal</td></tr><tr><td>C57BL/6J female mice</td><td>The Jackson Laboratory</td><td>000664</td><td>10-12 semanas de idade, fêmeas virgens</td></tr><tr><td>C57BL/6J male mice </td><td>The Jackson Laboratory</td><td>000664</td><td>Macho reprodutor</td></tr><tr><td>C57BL/6NCrl female mice</td><td>Charles River Laboratories</td><td>027</td><td>10-12 semanas de idade, fêmeas virgens</td></tr><tr><td>Centrifuge (24-place lab standard)</td><td>Eppendorf</td><td>5425 R</td><td>Centrifugar sangue para coletar soro</td></tr><tr><td>Data documentation platform </td><td>Airtable, Microsoft Excel, etc.</td><td></td><td>Mantenha registros de dados importantes, como pesos, hora da injeção, etc. (Tabela 1)</td></tr><tr><td>Digital dry bath - One block</td><td>Medicus Health</td><td>5277M1</td><td>Use para aquecer PIC antes da injeção</td></tr><tr><td>Eppendorf tubes - 1.5 mL</td><td>Eppendorf</td><td>30123611</td><td>Use ao diluir PIC para concentrações de estoque e de trabalho</td></tr><tr><td>Fisherbrand gauze sponges</td><td>Fisher Scientific</td><td>MSD-1400250</td><td>Use para parar o sangramento da cauda após a coleta de sangue</td></tr><tr><td>Fisherbrand mall probe 6 in.</td><td>Fisher Scientific</td><td>13-820-026</td><td>Use para verificação de obstrução</td></tr><tr><td>HydroGel</td><td>Clear H20</td><td>70-01-5022</td><td>Suplemento após injeções</td></tr><tr><td>Mouse IL-6 uncoated ELISA kit</td><td>Thermo Fisher Scientific</td><td>88-7064-88</td><td>Quantificar níveis de IL-6 no soro materno</td></tr><tr><td>Nalgene polypropylene Griffin low-form plastic beakers</td><td>Thermo Fisher Scientific</td><td>1201-1000PK</td><td>Coloque os ratos no copo ao pesar</td></tr><tr><td>Poly(I:C) (LMW)</td><td>Invivogen</td><td>tlrl-picw</td><td>PIC (LMW) para injeção MIA</td></tr><tr><td>Polyinosinic–polycytidylic acid sodium salt</td><td>Sigma</td><td>P9582</td><td>PIC (Sigma) para injeção MIA</td></tr><tr><td>Scalpel blades, #22, 10 pack</td><td>Home Science Tools</td><td>DE-SC22BLD</td><td>Use para cortar a ponta da cauda para coleta de sangue</td></tr><tr><td>Sodium chloride 0.9% (Normal saline), USP, Sterile grade, Cytiva Hyclone</td><td>Cytiva</td><td>Z1376</td><td>Injete para a geração de camundongos de controle</td></tr><tr><td>Tail snipping platform</td><td>Braintree Scientific, Inc.</td><td>IL-200</td><td>Restrainha adequadamente os camundongos durante os sangramentos da cauda</td></tr><tr><td>Vortex-Genie 2</td><td>Scientific Industries, Inc.</td><td>SI-0236</td><td>Vortex PIC após a diluição</td></tr></tbody></table>

geração do modelo de camundongo de ativação imunológica materna gestacional precoce para avaliar a inflamação pré-natal no neurodesenvolvimento

Generation of the Early-Gestational Maternal Immune Activation Mouse Model to Assess Prenatal Inflammation on Neurodevelopment

Geração do modelo de camundongo de ativação imunológica materna gestacional precoce para avaliar a inflamação pré-natal no neurodesenvolvimento

Neurodevelopmental disorders (NDDs) arise from complex interactions between genetic and environmental risk factors, including prenatal immune activation. ...

generation-early-gestational-maternal-immune-activation-mouse-model

Research

JoVE Journal

Neuroscience

Neurociência

A encefalopatia da prematuridade engloba as anormalidades do sistema nervoso central associadas à lesão do parto prematuro. Este relatório descreve um modelo de rato clinicamente relevante de hipóxia-isquemia sistêmica transitória in utero e administração intra-amniótica de lipopolissacarídeo (LPS) que imita a corioamnionite e o impacto relacionado de estímulos infecciosos e subperfusão placentária no desenvolvimento do SNC.

Modelagem Encefalopatia da Prematuridade Usando pré-natal hipóxia-isquemia com Lipopolysaccharide intra-amniótica em Ratos

A ativação imune materna (MIA) é um modelo para um fator de risco ambiental de autismo e esquizofrenia. O objetivo deste artigo é fornecer um procedimento passo a passo de como induzir MIA em camundongos prenhes, a fim de aumentar a reprodutibilidade deste modelo.

Indução de Maternal Immune Activation em Ratos na Fase Mid-gestação com Viral Mimic poli (I: C)

Desenvolvemos um modelo de coioamnionite para simular a exposição fetal à inflamação materna (FEMI) sem complicações de organismos vivos para examinar os efeitos do FEMI no desenvolvimento do trato intestinal da prole. Isso permite o estudo de causas mecanicistas para o desenvolvimento de lesões intestinais após a coioamnionite.

Um modelo murino de exposição fetal à inflamação materna para estudar os efeitos da coioamnionite aguda no desenvolvimento intestinal recém-nascido

O paradigma do estresse psicossocial crônico (CGS) emprega estressores clinicamente relevantes durante a gravidez em camundongos para modelar transtornos psiquiátricos de mães e bebês. Aqui, fornecemos um procedimento passo-a-passo de aplicação do paradigma CGS e avaliações a jusante para validar esse modelo.

Usando um modelo murino de estresse psicossocial na gravidez como um paradigma transcanalmente relevante para transtornos psiquiátricos em mães e bebês

A infecção materna é um fator de risco para distúrbios do neurodesenvolvimento. Modelos de camundongos de ativação imune materna (MIA) podem elucidar o impacto da infecção no desenvolvimento e função cerebral. Aqui, diretrizes gerais e um procedimento são fornecidos para produzir descendentes confiáveis e suscetíveis expostos ao MIA.

Gerando um Modelo Reprodutível de Ativação Imune Materna de Gestações Médias usando Poly(I:C) para Estudo de Suscetibilidade e Resiliência em Descendentes

O parto prematuro (parto &lt; 37 semanas) é um problema de saúde global urgente com opções de prevenção e tratamento abaixo do ideal. Apresentamos um modelo de camundongo de parto prematuro induzido por infecção bacteriana vaginal ascendente e descrevemos como analisar a morbidade e mortalidade neonatal resultante.

Modelagem de infecção vaginal ascendente, parto prematuro e morbidade neonatal em camundongos

Enxaqueca e sua transformação para enxaqueca crônica são um fardo imenso de saúde que precisam de opções de tratamento melhoraram. Procuramos definir como sinalização imune neural modula a suscetibilidade à enxaqueca,...

Modelagem Neural Sinalização Imune de migrânea episódica e crônica Usando a depressão alastrante In Vitro</em

Um paradigma lesão utilizando a Drosophila larval cordão nervoso ventral para investigar a regeneração do sistema nervoso central e de reparação é descrito. Stabbing seguido por varredura a laser microscopia confocal em amostras de lapso de tempo e fixa, combinada com a análise quantitativa, com software desenvolvido propositadamente e genética, são utilizados para investigar os mecanismos moleculares do CNS regeneração e reparo.

Um Paradigma para Investigar Lesão de reparação do sistema nervoso central em<em&gt; Drosophila</em

Gânglios da raiz dorsal (DRG) são estruturas que contêm os neurónios sensoriais do sistema nervoso periférico. Quando dissociada, podem ser co-cultivadas com células estaminais do tipo SC, derivadas de tecido adiposo (ASC), proporcionando um valioso modelo para estudar a regeneração de nervos em vitro e mielinização, simulando o ambiente in vivo, no local da lesão.

Dorsal Root Ganglia neurônios e células-tronco diferenciadas adiposo-derivados: An<em&gt; In Vitro</em&gt; Co-cultura modelo para estudar a regeneração do nervo periférico

Aqui, apresentamos um protocolo para infusão cerebral estereotáxica direta de beta-amilóide. Esta metodologia fornece um modelo alternativo de camundongo in vivo para abordar os efeitos de curto prazo da beta-amilóide nos neurônios cerebrais.

Infusão estereotáxica de oligomica beta-amilóide no ratinho Hippocampus

Aprenda a gerar um modelo de ativação imunológica materna em camundongos durante a gestação precoce para avaliar a inflamação pré-natal no neurodesenvolvimento. Este protocolo padronizado melhora a reprodutibilidade.

Geração do modelo de camundongo de ativação imunológica materna gestacional precoce para avaliar a inflamação pré-natal no neurodesenvolvimento (Vídeo) | JoVE Journal

Geração do modelo de camundongo de ativação imunológica materna gestacional precoce para avaliar a inflamação pré-natal no neurodesenvolvimento

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