Name: Detecção de Senescência usando luz refletida na fração vascular estromal adiposa (Vídeo) | JoVE Journal
Uploaded: 2026-06-05T13:15:00.000+00:00
Duration: 10 min 8 s
Description: Aprenda a detectar senescência celular em fração vascular estromal adiposa humana usando microscopia confocal de luz refletida. Este método quantitativo permite análise de células individuais e imunocitoquímica multiplexada.

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</ol>

<table><tbody><tr><td>1.5 mL tubes</td><td>BioSphere</td><td>72.706.200</td><td>Biosphere safeseal tube 1.5 mL, 250 PC</td></tr><tr><td>5 mL tubes</td><td>Eppendorf</td><td>30119401</td><td>Eppendorf&nbsp;tubes&nbsp;5.0&nbsp;mL microtube</td></tr><tr><td>96-well plate</td><td>Corning</td><td>4680</td><td>Corning 96-well high content ccreening microplates with film bottom</td></tr><tr><td>CD31 primary antibody</td><td>Abcam</td><td>Ab9498</td><td>Anti-CD31 antibody clone JC/70A</td></tr><tr><td>Confocal microscope with reflected light capabilities</td><td>Evident</td><td>FV-4000</td><td></td></tr><tr><td>Coverslips</td><td>Epredia</td><td>BB02400500A113MNZO</td><td>Coverslips 24 mm x 50 mm, #1.5 thickness (0.13-0.16 mm)&nbsp;</td></tr><tr><td>DPBS</td><td>Gibco</td><td>14190-136</td><td>DPBS, no calcium, no magnesium</td></tr><tr><td>Fixation buffer</td><td>Sigma Aldrich</td><td>1.00496.5000</td><td>Formaldehyde solution 4%, buffered, pH 6.9&nbsp;</td></tr><tr><td>HCl</td><td>Sigma-Aldrich</td><td>320331-500mL</td><td>Hydrochloric acid (37%)</td></tr><tr><td>Incubator</td><td>Stuart</td><td>S130H</td><td>Hybridization oven/shaker</td></tr><tr><td>Microscopy slides</td><td>Avantor VWR</td><td>631-1560</td><td>Single-frosted slides, coloured ends</td></tr><tr><td>Mounting media</td><td>Invitrogen</td><td>P36984</td><td>ProLong glass antifade mountant excitation/emission max 361/497 nm</td></tr><tr><td>Mouse IgG1&nbsp;isotype control</td><td>R&amp;D Systems</td><td>MAB002</td><td></td></tr><tr><td>NaOH</td><td>Sigma-Aldrich</td><td>S8045-500G</td><td>Sodium hydroxide &ge; 98%</td></tr><tr><td>Normal donkey serum</td><td>Jackson ImmunoResearch Labs</td><td>017-000-121</td><td></td></tr><tr><td>pH meter</td><td>MeterLab</td><td>R21M002&nbsp;</td><td>PHM210 standard pH meter&nbsp;</td></tr><tr><td>Pipette tips (1000 &micro;L)</td><td>Biosphere</td><td>70.1186.210</td><td>1250&micro;L filtered pipette tips</td></tr><tr><td>Pipette tips (200 &micro;L)</td><td>Corning</td><td>4823</td><td>200&micro;L filtered pipette tips</td></tr><tr><td>SABG Kit</td><td>Cell signaling</td><td>9860S</td><td>Senescence beta-galactosidase staining kit</td></tr><tr><td>Secondary antibody, Alexa Fluor 555</td><td>Invitrogen</td><td>A31570</td><td>Donkey anti-mouse IgG (H+L) highly cross-adsorbed excitation/emission max 553/568 nm</td></tr><tr><td>Tween-20</td><td>Fisher Scientific</td><td>10419000</td><td>Pure, Fisher chemical</td></tr><tr><td>Water bath</td><td>Rowe Scientific</td><td>SWB20D</td><td>Standard water bath</td></tr><tr><td>WGA</td><td>Invitrogen</td><td>W11261</td><td>Wheat Germ Agglutinin (WGA) Alexa Fluor 488 conjugate: Excitation/emission max 495/519 nm</td></tr></tbody></table>

detecção de senescência usando luz refletida na fração vascular estromal adiposa

Senescence Detection Using Reflected Light in Adipose Stromal Vascular Fraction

Detecção de Senescência usando luz refletida na fração vascular estromal adiposa

Cellular senescence is a stress-induced state characterized by permanent cell-cycle arrest and the development of a distinctive secretory profile that ...

senescence-detection-using-reflected-light-adipose-stromal-vascular

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JoVE Journal

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Biologia

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Aprenda a detectar senescência celular em fração vascular estromal adiposa humana usando microscopia confocal de luz refletida. Este método quantitativo permite análise de células individuais e imunocitoquímica multiplexada.

Detecção de Senescência usando luz refletida na fração vascular estromal adiposa

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