Super-Resolution Imaging бактериального Машины отдела
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Summary
Abstract
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| 50 x MEM Amino Acids | Sigma | M5550 | |
| 100 x MEM Vitamins | Sigma | M6895 | |
| IPTG | Mediatech | 46-102-RF | |
| 16% Paraformaldehyde | Electron Micrsocopy Sciences | 15710-S | |
| SeaPlaque GTG Agarose | Lonzo | 50111 | |
| 50 nm Gold Beads | Microspheres-Nanospheres | 790113-010 | |
| FCS2 Imaging Chamber | Bioptechs | ||
| Stage Adaptor | ASI | I-3017 | |
| Inverted Microscope | Olympus | IX71 | |
| 1.45 NA, 60x Objective | Olympus | ||
| IXON EMCCD Camera | Andor Technology | DU897E | |
| 488-nm Sapphire Laser | Coherent | ||
| 561-nm Sapphire Laser | Coherent | ||
| 405-nm CUBE Laser | Coherent |
References
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Cite This Article
Buss, J., Coltharp, C., Xiao, J. Super-resolution Imaging of the Bacterial Division Machinery. J. Vis. Exp. (71), e50048, doi:10.3791/50048 (2013).