Bacterial Colonization
Bacterial Colonization - Related Videos
02:32
Monitoring Bacterial Colonization of Arabidopsis thaliana Roots in a Soilless System
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2026
Source: Harris, S. L., et al. Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System. J. Vis. Exp. (2019)This video demonstrates a protocol for monitoring the colonization of root-associated bacteria on Arabidopsis thaliana seedlings grown in a soilless system. Following colonization, bacteria are dislodged by sonication, then diluted, plated, and incubated to form colonies that reflect bacterial attachment and viability.
02:43
Fluorescence Imaging of Bacterial Colonization in Mouse Kidney Tissue
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2026
Source: Behera, R. K., et al. A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues. J. Vis. Exp. (2019)This video demonstrates a method for preparing infected mouse kidney tissue, staining DNA, and detecting bacterial gene expression using dual fluorescent reporters. Red marks all bacteria, and green highlights infection-responsive cells.
11:18
Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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Cited by 1 •
2014
The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.
01:32
Enhancing Salt Stress Tolerance in Tomato Plants Through Endophytic Bacterial Colonization
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2025
Endophytic bacteria colonize plant tissues and promote plant growth.To study their impact on tomato plant growth under salt stress, start with sterile seedlings showing developing leaves and roots.Immerse the roots of the experimental seedling in a suspension of endophytic bacteria; leave the control uninoculated.Transfer both groups into sterilized soil to prevent external microbial interference and incubate in a growth chamber.From the soil, the bacteria enter through transient root openings,...
03:22
Evaluating Intestinal Bacterial Colonization in Germ-Free C. elegans
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2025
Source: Taylor, M. N., et.al. Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions. J. Vis. Exp. (2022)This video demonstrates the exposure of germ-free, age-matched Caenorhabditis elegans worms to a bacterial strain for intestinal colonization. It explains the selective removal of non-adhered and external bacteria, leaving only viable bacteria adhered to the gut epithelium for quantification.
FAQs
Persistence depends on successful arrival, adherence to epithelial surfaces or medical devices, and multiplication in conditions that support survival. The surrounding microbiota and host immune system also shape whether organisms remain established. These interacting factors explain why the same bacterial presence can remain clinically quiet in one setting yet become relevant when host or local conditions change.
Biofilms can give attached bacteria a protective community structure, allowing them to persist on epithelial surfaces or medical devices. Their presence matters clinically because device-associated colonization may complicate assessment of infection risk and prevention. Recognizing this mechanism helps researchers and clinicians focus on how attachment and persistence contribute to later clinical concerns.
Resident microbial communities and the host immune system are active parts of the colonization environment. They can influence whether bacteria survive and remain established, rather than merely serving as background conditions. Studying these interactions helps explain how colonization relates to health and disease and why its clinical significance varies among patients.
The result should be considered in relation to whether there is evidence of tissue damage or clinical disease, rather than treated as proof of infection by itself. This distinction supports more accurate assessment of transmission and infection risk and can prevent unnecessary antibiotic treatment when colonization is the better interpretation.
Detection on a device can identify an important site where bacteria adhere, multiply, and potentially form protective biofilms. This information supports strategies designed to prevent device-associated infections. It also gives clinicians a basis for considering device-related infection risk when interpreting microbiological findings, rather than viewing the culture in isolation.
In vulnerable patients, colonization information can contribute to decisions about infection risk, transmission, and clinical management. It may help teams distinguish a finding that reflects established microbial presence from one requiring concern about disease, while supporting targeted prevention efforts. This is particularly relevant when unnecessary antibiotic treatment could be avoided.