Atp Utilization Assay
Atp Utilization Assay - Related Videos
02:56
An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment
0 Views •
2025
This video describes the simple ATP-measuring assay using luciferase glow reagent. This assay is used to quantify Neisseria gonorrhoeae survival after treatment with ceftriaxone.
08:32
The ATP Bioluminescence Assay
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2023
In fireflies, the luciferase enzyme converts a compound called luciferin into oxyluciferin, and produces light or “luminescence” as a result. This reaction requires energy derived from ATP in order to proceed, so researchers have exploited the luciferase-luciferin interaction to gauge ATP levels in cells. Given ATP’s role as the cell’s currency of energy, the ATP bioluminescence assay can provide insight into cellular metabolism and overall cell health.In this video, JoVE discusses cellular...
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
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Cited by 15 •
2017
Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.
01:31
ATP Yield
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2019
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle). The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
01:08
Hydrolysis of ATP
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2019
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids. If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine monophosphate—by the removal of a second...
FAQs
The assay links ATP depletion or consumption to a coupled luminescent or colorimetric readout. An enzyme, cell extract, or pathogen-associated reaction receives ATP, and the resulting signal is evaluated against appropriate controls to estimate how much ATP the system used. This design connects a biochemical energy requirement with an experimentally measurable change.
Controls establish the reference signal needed to distinguish ATP consumption by the test system from changes unrelated to the reaction. Comparing the measured luminescent or colorimetric response with these controls supports interpretation of ATP depletion and helps determine whether differences reflect altered enzyme activity, cellular responses, or pathogen-associated metabolism.
The assay format can be applied to purified enzymes, cell extracts, and pathogen-associated reactions, allowing ATP use to be studied at different biological levels. In immunology and infection research, this range supports analysis of energy-dependent molecular activity as well as broader changes associated with immune cells, microbes, or interactions between hosts and pathogens.
A typical workflow supplies ATP to the selected enzyme, cell extract, or pathogen-associated reaction, allows the relevant ATP-dependent process to occur, and then measures ATP depletion or consumption through a coupled luminescent or colorimetric signal. The result is interpreted relative to appropriate controls, producing a comparative measure of energy use.
Changes in ATP use can provide information about energy-dependent immune-cell responses. In an immunology setting, researchers can compare ATP consumption across experimental conditions to characterize activation-associated metabolic changes or ATP-dependent signaling. The assay therefore offers a biochemical readout that complements broader studies of immune-cell function and response.
In infection research, ATP consumption measurements can characterize microbial metabolism, pathogen viability, and ATP-dependent host-pathogen interactions. Comparing reactions or conditions may reveal altered energy use associated with a pathogen or with an intervention. The same approach can support evaluation of potential antimicrobial or immunomodulatory effects when those treatments change ATP utilization.