Ha Antibody Assay
Ha Antibody Assay - Related Videos
03:03
Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies
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2025
This video demonstrates an assay measuring the antibody-mediated activation of T cell effector functions. Adding an influenza virus hemagglutinin (HA)-specific monoclonal antibody to transfected mammalian cells expressing HA results in the formation of immune complexes with HA. Upon introducing engineered T cells expressing Fc receptors and a luciferase reporter, the T cells are activated by the antibody-HA complexes, and this activation is detected by adding a luciferase substrate and...
13:12
Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
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Cited by 19 •
2011
A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.
05:22
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
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2025
Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.
06:08
Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies
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2023
This protocol describes the application of a novel hybrid alphavirus-SARS-CoV-2 pseudovirus (Ha-CoV-2) as a platform for rapid quantification of infectivity of SARS-CoV-2 variants and their sensitivity to neutralizing antibodies.
04:20
Functional Assessment of Antibody-Secreting B Cells Using ELISpot Assay
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2025
This video demonstrates the use of the ELISpot assay to assess the function of antibody-secreting B cells, ASCs. The antibodies secreted by the ASCs interact with the membrane-bound polyclonal antibody fragments, which are detected by the enzyme-conjugated IgG or IgM antibodies. The colored spot produced in each well indicates the presence of the specific antibody's isotype, which correlates with the B cells' secretory activity.
FAQs
Antibody activity is reflected by the extent to which serum prevents HA from producing red blood cell clumping. When antibodies bind the viral surface protein effectively, the interaction between HA and red blood cell receptors is reduced. Greater inhibition therefore indicates stronger blocking activity in the tested serum, allowing researchers to quantify or compare immune responses.
Hemagglutinin is the viral surface protein responsible for attachment and entry, so antibodies that recognize it can interfere with an early step of infection. Measuring this activity connects the assay result to a specific host-pathogen interaction rather than to antibodies in general. The assay can therefore help examine how immune recognition relates to viral infectivity.
Differences in inhibition can indicate that sera recognize viral HA with different effectiveness. Comparing responses against distinct viral antigens helps researchers evaluate antigenicity, meaning the features that influence immune recognition. This information is useful for characterizing evolving viral strains and determining whether an immune response reacts similarly across the viral materials being compared.
The assay uses serum containing antibodies, active HA, and red blood cells with receptors that can interact with the viral protein. Researchers assess whether visible cell clumping occurs or is inhibited after antibody binding. This observable change provides the basis for estimating antibody activity and linking the experimental result to HA-mediated attachment.
Serum collected after vaccination can be examined for its ability to inhibit HA-mediated red blood cell clumping. The resulting measurement helps characterize the antibody response generated by the vaccine and supports comparisons among immune responses. In infection research, these data contribute to assessing whether vaccine-induced antibodies recognize and block a relevant viral surface function.
Testing serum from naturally infected or otherwise exposed hosts can show whether acquired antibodies inhibit the relevant HA interaction. Researchers can compare these findings with vaccine-induced responses and with results from different viral strains. Such comparisons help characterize protection-related immune activity, monitor antigenic change, and inform surveillance of evolving viruses.