Fundus Imaging

Fundus imaging is a noninvasive technique that captures photographs of the interior surface of the eye, including the retina, optic disc, macula, and retinal blood vessels. It works by directing light through the pupil and recording the light reflected from these structures with a specialized fundus camera, producing images that can reveal changes in tissue appearance and vascular patterns. In neuroscience, fundus imaging supports assessment of the visual system and provides an accessible view of neural tissue and its blood supply. It is used in research and clinical evaluation of optic nerve disorders, retinal disease, and neurodegenerative conditions associated with vascular or retinal changes.

Fundus Imaging - Related Videos

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JoVE Journal - Medicine
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Smartphone Fundus Photography

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Cited by 49 •

2017

Fundus photography normally requires specialized fundus cameras that are not always available in all clinical settings. Here, a simple method to record ocular fundus images using a smartphone camera and a conventional high-plus handheld indirect ophthalmoscopy lens is described.

Research

JoVE Journal - Medicine

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

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Cited by 22 •

2016

The retinal pigment epithelium (RPE) supports the sensory retina through recycling visual cycle byproducts, which accumulate as lipofuscin. These products are autofluorescent and can be qualitatively imaged in vivo. Here, we describe a method to quantitatively image RPE lipofuscin using confocal scanning laser ophthalmoscopy.

Using Retinal Imaging to Study Dementia

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Cited by 19 •

2017

The retina shares prominent similarities with the brain and thus represents a unique window to study vasculature and neuronal structure in the brain non-invasively. This protocol describes a method to study dementia using retinal imaging techniques. This method can potentially aid in diagnosis and risk assessment of dementia.

Research

JoVE Journal - Medicine
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A Workflow to Quantitatively Determine Age-Related Macular Degeneration Lesion-Specific Variations in Fundus Autofluorescence

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Cited by 2 •

2023

This research describes a workflow to determine and compare autofluorescence levels from individual regions of interest (e.g., drusen and subretinal drusenoid deposits in age-related macular degeneration [AMD]) while accounting for varying autofluorescence levels throughout the fundus.

In Vivo Imaging of Retinal Microglia in a Mouse Model of Glaucoma

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2025

Source: Bosco, A., et al. In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma. J. Vis. Exp. (2015) This video demonstrates in vivo imaging of retinal microglia in a mouse model of glaucoma. A transgenic mouse with fluorescent protein-expressing retinal microglia is positioned under an ophthalmoscope. Infrared illumination is used to locate the optic nerve head (ONH), and the focal plane is...

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