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November 30, 2014
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RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.
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Poelchau, M. F., Huang, X., Goff, A., Reynolds, J., Armbruster, P. An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus. J. Vis. Exp. (93), e51961, doi:10.3791/51961 (2014).
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