Three-dimensional Co-culture Model for Tumor-stromal Interaction

Masafumi Horie; Akira Saito; Yoko Yamaguchi; Mitsuhiro Ohshima; Takahide Nagase

doi:10.3791/52469

Журнал

/

Медицина

/

Трехмерная Co-культура Модель опухолью стромы взаимодействия

JoVE Journal
Медицина

Для просмотра этого контента требуется подписка на Jove Войдите в систему или начните бесплатную пробную версию.

JoVE Journal Медицина

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Трехмерная Co-культура Модель опухолью стромы взаимодействия

Please note that all translations are automatically generated. Click here for the English version.

17,246 Views

•

08:39 min

•

February 02, 2015

DOI:

10.3791/52469-v

DOI:

10.3791/52469-v

•

08:39 min

February 02, 2015

•

5 Views

Masafumi Horie^1,2, Akira Saito^1,3, Yoko Yamaguchi⁴, Mitsuhiro Ohshima⁵, Takahide Nagase¹

¹Department of Respiratory Medicine, Graduate School of Medicine,The University of Tokyo, ²Department of Clinical Laboratory, Graduate School of Medicine,The University of Tokyo, ³Division for Health Service Promotion,The University of Tokyo, ⁴Department of Biochemistry,Nihon University School of Dentistry, ⁵Department of Biochemistry,Ohu University School of Pharmaceutical Sciences

Резюме

Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.