Оценка эффективности Н. пилори Белок HP-NAP как лечебное средство для лечения рака мочевого пузыря в Ортотопическая мышиной модели

Overview

This article describes a method to establish a syngeneic mouse model of orthotopic bladder tumor for evaluating the anti-tumor efficacy of the bacterial protein HP-NAP. The procedure involves tumor cell injection into the bladder cavity and subsequent therapeutic agent delivery.

Key Study Components

Area of Science

• Oncology
• Immunology
• Mouse Models

Background

• Establishing tumor models is crucial for cancer research.
• Orthotopic models provide a more accurate representation of tumor behavior.
• HP-NAP is a bacterial protein with potential therapeutic effects.
• Histological analysis is essential for evaluating treatment efficacy.

Purpose of Study

• To create a reliable syngeneic mouse model for bladder tumors.
• To assess the therapeutic potential of HP-NAP in treating bladder cancer.
• To utilize histological methods for evaluating treatment outcomes.

Methods Used

• Harvesting tumor cells from culture and resuspending in PBS.
• Catheterizing female mice for tumor cell injection.
• Administering therapeutics three days post-tumor implantation.
• Performing histological and immunohistochemical analyses post-treatment.

Main Results

• Successful establishment of bladder tumors in the mouse model.
• Therapeutic agents showed varying levels of efficacy.
• Histological analysis confirmed the presence of tumors and treatment effects.
• Immunohistochemical analysis provided insights into immune responses.

Conclusions

• The syngeneic mouse model is effective for studying bladder tumors.
• HP-NAP demonstrates potential as a therapeutic agent.
• Histological methods are crucial for evaluating treatment efficacy.

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