CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Rafael D. Melani; Henrique S. Seckler; Owen S. Skinner; Luis H. F. Do Vale; Adam D. Catherman; Pierre C. Havugimana; Marcelo Valle de Sousa; Gilberto B. Domont; Neil L. Kelleher; Philip D. Compton

doi:10.3791/53597

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CN-GELFrEE - Clear Native гель-элюированный жидкая фракция Entrapment электрофорез

CN-GELFrEE – Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

JoVE Journal
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JoVE Journal Химия

CN-GELFrEE – Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

CN-GELFrEE - Clear Native гель-элюированный жидкая фракция Entrapment электрофорез

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11,746 Views

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11:38 min

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February 29, 2016

DOI:

10.3791/53597-v

DOI:

10.3791/53597-v

•

11:38 min

February 29, 2016

•

11728 Views

Rafael D. Melani*^1,2, Henrique S. Seckler*¹, Owen S. Skinner¹, Adam D. Catherman¹, Pierre C. Havugimana¹, Gilberto B. Domont², Neil L. Kelleher¹, Philip D. Compton¹

¹Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center,Northwestern University ²Institute of Chemistry, Proteomics Unit,Federal University of Rio de Janeiro ³Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry,University of Brasilia

Резюме

This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.