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June 03, 2016
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Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.
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Brinkmann, A., Okom, C., Kludt, E., Schild, D. Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis. J. Vis. Exp. (112), e54108, doi:10.3791/54108 (2016).
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