Помощью лазера цитоплазматических микроинъекции в животноводстве зиготы

Overview

This protocol demonstrates the technique of cytoplasmic microinjection in farm animal zygotes, enabling the delivery of various solutions, including genome editing tools, into one-cell embryos.

Key Study Components

Area of Science

• Animal Biotechnology
• Transgenesis
• Genome Editing

Background

• Cytoplasmic microinjection is a method used to introduce substances into zygotes.
• This technique is particularly useful in livestock research.
• Genome editing tools like CRISPR Cas9 can be delivered to create knockout animals.
• Efficient injection minimizes adverse effects on animal health.

Purpose of Study

• To effectively deliver solutions into the cytoplasm of livestock zygotes.
• To facilitate the creation of transgenic animals for research purposes.
• To improve the reliability of genome editing in large animals.

Methods Used

• Preparation of injection micropipette using a borosilicate glass capillary.
• Utilization of a micropipette puller to achieve a thin tip with a long taper.
• Application of a blunt-end needle for microinjection.
• Use of laser assistance to enhance injection precision.

Main Results

• The method allows for the successful delivery of solutions into zygotes.
• High injection efficiency was observed with minimal adverse effects.
• Demonstrated potential for creating genetically modified livestock.
• Provides a reliable approach for transgenesis in large animals.

Conclusions

• Cytoplasmic microinjection is an effective technique for genome editing in livestock.
• This method enhances the study of gene function in large animals.
• It offers a straightforward approach to producing transgenic animals.

Frequently Asked Questions