Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Volodymyr Petrenko; Camille Saini; Laurent Perrin; Charna Dibner

doi:10.3791/54673

Журнал

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Генетика

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Параллельное измерение циркадных часов экспрессии генов и гормональную секрецией человека первичных клеточных культур

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

JoVE Journal
Генетика

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JoVE Journal Генетика

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Параллельное измерение циркадных часов экспрессии генов и гормональную секрецией человека первичных клеточных культур

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8,173 Views

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06:53 min

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November 11, 2016

DOI:

10.3791/54673-v

DOI:

10.3791/54673-v

•

06:53 min

November 11, 2016

•

2 Views

Volodymyr Petrenko*¹, Camille Saini*², Laurent Perrin*¹, Charna Dibner¹

¹Department of Medical Specialties, Division of Endocrinology, Diabetes, Hypertension and Nutrition, Diabetes Center,University of Geneva Medical School, Institute of Genetics and Genomics in Geneva (iGE3), ²Population Epidemiology Unit (UEP), Community Medicine,Geneva University Hospital

ТРАНСКРИПТ

Резюме

Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.