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Пройдя через сигнализации различных белковых комплексов, очищение сродства Bimolecular дополнения (BiCAP)
Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
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JoVE Journal Иммунология и инфекции
Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Пройдя через сигнализации различных белковых комплексов, очищение сродства Bimolecular дополнения (BiCAP)

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Сгенерировано автоматически
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7,478 Views

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06:45 min

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June 15, 2018

DOI:

10.3791/57109-v

DOI:
10.3791/57109-v

•

06:45 min
June 15, 2018

•

7452 Views
, , , , , ,
1The Kinghorn Cancer Centre,Garvan Institute of Medical Research2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences,University of Copenhagen3RCSI Molecular Medicine,Royal College of Surgeons in Ireland4Department of Epigenetics,Max Planck Institute of Immunobiology and Epigenetics5School of Medical Sciences,University of New South Wales6St Vincent’s Hospital Clinical School,University of New South Wales7School of Medicine and Medical Science,University College Dublin

Резюме

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Эта рукопись описывает протокол для Bimolecular очищение сродства комплементарности (BiCAP). Этот новый метод облегчает конкретные изоляции и вниз по течению proteomic характеристик любых двух взаимодействующих белков, хотя исключая ООН complexed индивидуальных белков, а также комплексы с конкурирующими привязки партнерами.

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Hastings, J. F., Han, J. Z., Shearer, R. F., Kennedy, S. P., Iconomou, M., Saunders, D. N., Croucher, D. R. Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP). J. Vis. Exp. (136), e57109, doi:10.3791/57109 (2018).

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