Phosphopeptide обогащения, в сочетании с Label бесплатно количественного масс-спектрометрии для расследования Phosphoproteome рака простаты

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Цитируется: 15

12:23 мин.

2 августа 2018 г.

10.3791/57996-v

2 августа 2018 г.

14.1K просмотров

Larry C. Cheng^*¹^,² , Zhen Li^*³ , Thomas G. Graeber⁴ , Nicholas A. Graham⁵ , Justin M. Drake¹^,²^,³^,⁶^,⁷

¹Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, ²Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, ³Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, ⁴Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, ⁵Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, ⁶Pharmacology, Rutgers Robert Wood Johnson Medical School, ⁷Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey

^* These authors contributed equally

Этот протокол описывает процедуру для извлечения и обогатить phosphopeptides от линий клеток рака простаты или тканей для анализа phosphoproteome через массы на основе спектрометрии протеомики.