Выделение и анализ прослеживаемых и функционализированных внеклеточных везикул из плазмы и твердых тканей
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Цитируется: 7
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09:57 мин.
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17 октября 2022 г.
DOI :10.3791/63990-v
17 октября 2022 г.
•2.6K просмотров
Yuan Cao*1,2 , Ji-Yu Qiu*1,3 , Da Chen1,2 , Chen-Yao Li1,4 , Shu-Juan Xing1,5 , Chen-Xi Zheng1 , Xin Liu2 , Yan Jin1,6 , Bing-Dong Sui1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine
* These authors contributed equally
В настоящем протоколе описан способ извлечения внеклеточных везикул из периферической крови и солидных тканей с последующим профилированием поверхностных антигенов и белковых грузов.
Chapters in this video
0:05
Introduction
1:20
Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
2:50
Origin Analysis of Extracellular Vesicles
5:17
Protein Content Analysis of Extracellular Vesicles
8:06
Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
9:13
Conclusion
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