Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder

Tiange Xiao; Yueyi Chen; Alyssa Boisvert; Maury Cole; Adam Kimbrough

doi:10.3791/65320

Хроническое прерывистое воздействие паров этанола в сочетании с выбором двух бутылок для моделиро...

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Neuroscience

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JoVE Journal Neuroscience

Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder

Хроническое прерывистое воздействие паров этанола в сочетании с выбором двух бутылок для моделирования расстройства, связанного с употреблением алкоголя

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05:12 min

June 23, 2023

DOI: 10.3791/65320-v

Tiange Xiao¹, Yueyi Chen¹, Alyssa Boisvert¹, Maury Cole², Adam Kimbrough^1,3,4,5

¹Department of Basic Medical Sciences, College of Veterinary Medicine,Purdue University, ²The Scripps Research Institute, ³Purdue Institute for Integrative Neuroscience, ⁴Weldon School of Biomedical Engineering,Purdue University, ⁵Purdue Institute of Inflammation, Immunology, and Infectious Disease

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Overview

This study presents a protocol for the two-bottle choice/chronic intermittent ethanol (CIE) model of alcohol dependence in mice to investigate alcohol use disorder. The model leverages automated equipment to monitor mouse drinking behavior, providing insights into the neurobiological changes linked to alcohol dependence.

Key Study Components

Area of Science

Neuroscience
Behavioral Pharmacology
Alcohol Dependence Studies

Background

The two-bottle choice and CIE paradigm has been used to study alcohol dependence for many years.
Automated monitoring enables detailed analysis of drinking patterns beyond just volume.
This model can induce significant withdrawal symptoms and escalate alcohol intake.
Research in alcohol use disorder often employs techniques like imaging and genetic manipulation.

Purpose of Study

To provide an accessible protocol for studying alcohol dependence in mice.
To highlight the relevance of the two-bottle choice/CIE model in preclinical studies.
To encourage broader adoption of this methodology in alcohol research.

Methods Used

The main platform used is the two-bottle choice paradigm paired with CIE exposure.
Mice are individually housed and monitored for their alcohol intake over a specified period.
Baseline drinking is established before administering ethanol and CIE treatment.
Methods include intraperitoneal injections and blood ethanol concentration measurements.

Main Results

CIE exposure significantly increased ethanol intake in dependent mice over their baseline measurements.
Blood ethanol levels demonstrated that the CIE group maintained higher average concentrations compared to non-dependent mice.
Behavioral changes following CIE reflect crucial neurobiological alterations linked to alcohol dependence.

Conclusions

This study establishes the two-bottle choice/CIE model as a reliable technique for investigating alcohol dependence.
The methodology facilitates an in-depth examination of withdrawal symptoms and alcohol intake escalation.
Insights derived from this model can enhance our understanding of the neurobiological underpinnings of alcohol use disorders.

Frequently Asked Questions

What are the advantages of the two-bottle choice/CIE model?

This model allows for precise monitoring of drinking behavior and is capable of inducing significant withdrawal symptoms, making it relevant for studying alcohol use disorders.

How is the alcohol dependence model implemented in mice?

Mice are moved to individual cages, allowed to acclimate, and then given a choice between water and ethanol, followed by CIE exposure through vapor administration.

What types of data can be obtained from the study?

Data collected includes ethanol intake volumes, drinking patterns, and blood ethanol concentrations, providing insights into behavioral and physiological responses to alcohol.

How can this methodology be applied or adapted?

Researchers can adapt the protocol to explore various factors influencing alcohol consumption or to test pharmacological interventions impacting dependence.

What are the key limitations or considerations of this model?

The model may not fully replicate human alcohol use disorders, and individual variations in mouse behavior can affect reproducibility of results.

What insights does this study provide into neurobiological changes?

It highlights how chronic ethanol exposure can lead to significant changes in drinking behavior and blood ethanol levels, which relate to withdrawal and dependence mechanisms.

Здесь представлен протокол для модели алкогольной зависимости 2BC/CIE у мышей для изучения расстройств, связанных с употреблением алкоголя.

Наше исследование относилось к области расстройств, связанных с употреблением алкоголя. В данном исследовании освещается методология, обычно используемая в созданной модели Alco зависимости мыши. Эта модель представляет собой хронический прерывистый пар этанола в паре с парадигмой выбора двух бутылок.

Парадигма выбора двух бутылок CIE. Автоматизированное оборудование для выбора двух бутылок позволяет отслеживать поведение мыши при употреблении алкоголя, записывая частоту суперприкосновений. Это позволяет нам получать дополнительную информацию, выходящую за рамки потребляемого объема, например, время выпивки в течение сеанса выпивки.

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