Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model

Sijuan Sun; Hongbin Zhu; Shoubao Wang; Xiuxia Xu; Lincai Ye

doi:10.3791/65372

Создание и подтверждение постнатальной модели мышиной перегрузки объема правого желудочка

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JoVE Journal Medicine

Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model

Создание и подтверждение постнатальной модели мышиной перегрузки объема правого желудочка

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06:04 min

June 9, 2023

DOI: 10.3791/65372-v

Sijuan Sun*¹, Hongbin Zhu*², Shoubao Wang³, Xiuxia Xu⁴, Lincai Ye^2,5,6

¹Department of Pediatric Intensive Care Unit, Shanghai Children’s Medical Center, School of Medicine,Shanghai Jiaotong University, ²Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, School of Medicine,Shanghai Jiaotong University, ³Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine,Shanghai Jiao Tong University, ⁴Department of Radiology, Huangpu Branch, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine, ⁵Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, School of Medicine,Shanghai Jiaotong University, ⁶Shanghai Institute for Pediatric Congenital Heart Disease, Shanghai Children’s Medical Center, School of Medicine,Shanghai Jiaotong University

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Overview

This protocol presents the establishment and confirmation of a postnatal right ventricular volume overload (VO) model in mice with abdominal arteriovenous fistula (AVF), which can be applied to investigate how VO contributes to postnatal heart development.

Key Study Components

Area of Science

Congenital heart disease
Heart regeneration
Postnatal heart development

Background

Understanding the effects of volume or pressure overload on heart and lung development.
Development of various postnatal mouse or rat models of congenital heart disease.
Use of the AVF model to induce chronic heart VO in adult animals.
Limited knowledge about the AVF model in postnatal mice.

Purpose of Study

To investigate how volume overload affects postnatal heart development.
To advance understanding of pediatric congenital heart disease.
To establish a reliable model for future research.

Methods Used

Establishment of a postnatal right ventricular volume overload model.
Confirmation of the model's effectiveness.
Application of the model to study heart development.
Utilization of abdominal arteriovenous fistula (AVF) in mice.

Main Results

Successful establishment of the volume overload model in postnatal mice.
Insights into the impact of VO on heart development.
Potential for further research into pediatric heart conditions.
Contribution to the understanding of congenital heart disease mechanisms.

Conclusions

The AVF model is a valuable tool for studying postnatal heart development.
Volume overload has significant implications for heart health in pediatric populations.
Further research is needed to explore the long-term effects of VO.

Frequently Asked Questions

What is the significance of the AVF model?

The AVF model helps in understanding chronic heart volume overload in postnatal mice, which is crucial for studying congenital heart disease.

How does volume overload affect heart development?

Volume overload can lead to cardiomyopathy and affect the proliferation and maturation of heart cells during postnatal development.

What are the potential applications of this research?

This research can advance knowledge in pediatric congenital heart disease and inform treatment strategies.

Why is postnatal modeling important?

Postnatal modeling allows researchers to study heart development in a context that closely mimics human conditions.

What are the next steps in this research?

Future studies will explore the long-term effects of volume overload on heart function and development.

В этом протоколе представлено создание и подтверждение модели постнатальной объемной перегрузки (ВО) правого желудочка у мышей с брюшной артериовенозной фистулой (АВФ), которая может быть применена для изучения того, как ВО способствует постнатальному развитию сердца.

Область наших исследований – врожденные пороки сердца и регенерация сердца. Мы пытаемся понять, как объемная или перегрузка давлением влияет на постнатальное развитие сердца и легких, включая пролиферацию и созревание кардиомиоцитов. Мы разработали различные типы постнатальных моделей врожденных пороков сердца у мышей или крыс.

Например, перегрузка желудочков, перегрузка давлением, снижение легочного кровотока. Эти модели очень интересны и углубят наше понимание врожденных пороков сердца у детей. На протяжении десятилетий модель AVF использовалась для индуцирования хронического сердечного ВО у взрослых животных.

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