Using Human Intestinal Organoids to Understand the Small Intestine Epithelium at the Single Cell Transcriptional Level

Carolyn Bomidi; Xi-Lei Zeng; Victoria Poplaski; Cristian Coarfa; Mary K. Estes; Sarah E. Blutt

doi:10.3791/66749

Использование органоидов кишечника человека для понимания эпителия тонкой кишки на уровне транскр...

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Using Human Intestinal Organoids to Understand the Small Intestine Epithelium at the Single Cell Transcriptional Level

Использование органоидов кишечника человека для понимания эпителия тонкой кишки на уровне транскрипции отдельных клеток

Full Text

1,419 Views

08:23 min

June 28, 2024

DOI: 10.3791/66749-v

Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology,Baylor College of Medicine, ²Department of Molecular and Cellular Biology,Baylor College of Medicine

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study investigates the integration of single-cell transcriptomics and organoid technology to enhance understanding of small intestinal biology. The protocol enables unprecedented insight into the cellular heterogeneity within the intestinal epithelium, thereby advancing research on human intestinal diseases.

Key Study Components

Research Area

Understanding small intestinal biology
Identifying biomarkers for intestinal diseases
Advancing personalized medicine approaches

Background

Integration of organoid technology with transcriptomic analysis
Challenges in single-cell analysis and organoid research
Importance of cost reduction and improved computational methods

Methods Used

Single-cell transcriptomic analysis
Human intestinal organoids
Enzymatic cell digestion and cell suspension techniques

Main Results

Isolation of significant numbers of single cells from organoids
Insights into transcriptional landscapes and cell types in the intestinal epithelium
Potential for identifying biomarkers related to human diseases

Conclusions

Demonstrates the utility of combining organoid technology with transcriptomics for biological insights
Highlights relevance for advancing research on intestinal health and disease

Frequently Asked Questions

What are human intestinal organoids?

Human intestinal organoids are three-dimensional structures derived from intestinal stem cells that replicate the cellular architecture and function of the intestines.

How does single-cell transcriptomics enhance research?

Single-cell transcriptomics allows researchers to analyze gene expression at the individual cell level, revealing cellular heterogeneity and specific cell types within tissues.

What are the challenges in using organoids?

Challenges include limited spatial context, transcriptional variations during sample preparation, and understanding temporal dynamics of cellular functions.

How can this research aid in personalized medicine?

By identifying specific biomarkers and mechanisms associated with diseases, researchers can tailor treatments to individual patients, improving health outcomes.

What technological innovations are important in this study?

Innovations include improved computational methods and tools that increase the throughput and sensitivity of transcriptomic analyses.

What role do computational methods play?

Computational methods enhance the analysis of single-cell data, enabling researchers to interpret complex biological information more effectively.

Can this approach be applied to other organs?

Yes, the integration of organoid technology with single-cell analysis can potentially be applied to various organs to study their unique cellular dynamics.

Протокол сочетает в себе технологию органоидов кишечника человека с транскриптомным анализом отдельных клеток, чтобы обеспечить значительное понимание ранее неизученной биологии кишечника.

Мы интегрируем транскриптомику одиночных клеток и технологию органоидов, чтобы лучше понять биологию тонкой кишки. Эти технологии позволяют нам глубже понять клеточную гетерогенность, которая существует в этой ткани. Инструменты, повышающие пропускную способность, чувствительность и разрешение, являются ключом к дальнейшим исследованиям в этой области.

В качестве примера можно привести усовершенствованные вычислительные методы и технологические инновации. Снижение затрат также является одним из основных направлений. Процесс подготовки и секвенирования библиотек образцов может привести к вариациям транскрипции, что является одной из проблем.

View the full transcript and gain access to thousands of scientific videos