Isolation and Analysis of Aortic Arch and Root Lesions in an Atherosclerotic Mouse Model

Yu Yan; Guofang Wang; Longhua Liu

doi:10.3791/67875

Выделение и анализ поражений дуги аорты и корней на модели атеросклеротической мыши

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JoVE Journal Medicine

Isolation and Analysis of Aortic Arch and Root Lesions in an Atherosclerotic Mouse Model

Выделение и анализ поражений дуги аорты и корней на модели атеросклеротической мыши

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07:49 min

February 14, 2025

DOI: 10.3791/67875-v

Yu Yan*¹, Guofang Wang*¹, Longhua Liu¹

¹School of Exercise and Health,Shanghai University of Sport

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Overview

This protocol provides a comprehensive methodology for the quantitative analysis of atherosclerotic plaques to facilitate more in-depth research into the mechanisms and progression of atherosclerosis. The study investigates atherosclerotic lesion development in LDLR knockout mice, detailing techniques for isolation, staining, and analysis.

Key Study Components

Area of Science

Neuroscience
Cardiovascular Biology
Pathology

Background

Atherosclerosis is a major contributor to cardiovascular diseases.
Understanding plaque composition is crucial for assessing disease severity.
LDLR knockout mice serve as a model for studying atherosclerosis.
Histological examination is a key method for analyzing plaques.

Purpose of Study

To provide a detailed protocol for analyzing atherosclerotic plaques.
To assess the severity and composition of atherosclerosis in a mouse model.
To enhance understanding of atherosclerosis mechanisms.

Methods Used

Isolation and staining of atherosclerotic plaques.
Histological examination of lesions.
Assessment of biochemical markers.
Vascular ultrasound techniques.

Main Results

Detailed methodology for plaque analysis was established.
Technical challenges in dissection procedures were identified.
Comprehensive assessment techniques were outlined.
Findings contribute to understanding atherosclerosis progression.

Conclusions

The protocol aids in the quantitative analysis of atherosclerotic plaques.
Improved techniques can enhance research into cardiovascular diseases.
Further studies are needed to explore underlying mechanisms.

Frequently Asked Questions

What is the significance of studying atherosclerosis?

Atherosclerosis is a leading cause of cardiovascular diseases, making its study crucial for developing effective treatments.

How does the LDLR knockout mouse model contribute to this research?

LDLR knockout mice are used to mimic human atherosclerosis, allowing for detailed study of plaque development and progression.

What techniques are used for plaque analysis?

Techniques include histological examination, biochemical marker assessment, and vascular ultrasound.

What are the main challenges in the dissection procedure?

The precision required for in vivo aortic stripping poses significant technical hurdles.

What are the expected outcomes of this study?

The study aims to provide a comprehensive methodology for analyzing atherosclerotic plaques and understanding their progression.

How can this research impact cardiovascular disease treatment?

By improving understanding of atherosclerosis, this research can lead to better prevention and treatment strategies for cardiovascular diseases.

Этот протокол предоставляет всестороннюю методологию количественного анализа атеросклеротических бляшек, чтобы способствовать более глубокому исследованию механизмов и прогрессирования атеросклероза.

В исследовании изучается развитие атеросклеротического поражения в дуге аорты и корне нокаутированных мышей ЛПНП, предлагается протокол выделения, окрашивания и анализа для оценки состава бляшек тяжести атеросклероза. Помимо гистологического исследования, используемого в этом исследовании, практические методы оценки атеросклероза также включают биохимические маркеры, молекулярно-биологические тесты и ультразвуковое исследование сосудов. Точность процедуры диссекции представляет собой самое большое техническое препятствие при выполнении in vivo стриппинга аорты на мышиной модели атеросклероза.

Для начала поместите усыпленных мышей в лежачем положении на пенопластовую доску, покрытую одним-двумя слоями впитывающей бумаги. Зафиксируйте конечности булавками для стабилизации тела. Распылите 75% этанол на брюшную полость мышей, чтобы очистить и увлажнить шерсть.

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