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Analytical Chemistry
HPLC Separation in Soft Drink Analysis
Description
High-performance liquid chromatography (HPLC) is used to separate and measure components in liquid samples. It works by pumping a solution through a column packed with small porous particles. A second phase is bound to the particle surface inside the column. Because sample components dissolve differently in these two phases,...
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Transcript
High-performance liquid chromatography, or HPLC, is a highly versatile technique that separates components of a liquid mixture based on their different interactions with a stationary phase.
HPLC is an adaptation of column chromatography. In column chromatography, a column is packed with micro-scale beads called the stationary phase. The st...
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