Saponification for Cleaner GC-FID Analysis

Saponification helps clean up complex lipid samples before gas chromatography analysis. A total lipid extract, or TLE, from an organic solvent extraction can contain hundreds or even thousands of compounds. Researchers may only need a few target compounds. They may also need to remove unwanted material that gets in the way o...

Saponification is a technique commonly used to remove fatty acid methyl esters from a complex organic mixture.

Complex organic samples are often analyzed by gas chromatography, which is used to determine the relative concentrations of individual components.

However, compounds that are similar in size and structure cannot be distingui...