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Environmental Sciences

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Environmental Sciences

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Environmental Microbiology

qPCR for Detecting Waterborne Viruses

Description

Quantitative polymerase chain reaction (qPCR), also called real-time PCR, is a molecular method used to measure microorganisms in environmental samples. It helps researchers estimate how much microbial material is present, including disease-causing pathogens. This makes qPCR useful in environmental microbiology when samples ...

Transcript

The advent of quantitative polymerase chain reaction, or qPCR, has made it possible to quantitatively determine the amount of any microorganism in an environmental sample.

Like standard PCR, qPCR identifies microorganisms by detecting for the presence or absence of DNA sequences specific to the organisms of interest. This allows for the de...

Tags

Quantitative Polymerase Chain ReactionEnvironmental Microorganism DetectionVirus QuantificationReal Time PCRStandard Curve AnalysisThreshold Cycle MeasurementFluorescent Probe DetectionReverse TranscriptionSoil Sample ProcessingLive Dead Discrimination

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