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Biology

Science Experiments

Basic Biology

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Biology II: Mouse, Zebrafish, and Chick

Mouse Genotyping

Description

Even though the human genome was mapped over 10 years ago, scientists are still far from understanding the function of every human gene! One way to evaluate how a gene functions is to disrupt the sequence encoding it and then evaluate the impact of this change (the phenotype) on the animal’s biology. This approach is commonly used in the m...

Transcript

Genotyping is the process of detecting the presence, or absence, of specific DNA sequences in a particular organism’s genome.

Since genes can influence a mouse’s phenotype, being able to probe an individual mouse’s genetic make-up, or “genotype,” is critical for attributing a phenotype to a specific gene. This video wi...

Tags

Mouse GenotypingDNA ExtractionPCR AmplificationAgarose Gel ElectrophoresisTissue DigestionHomozygous KnockoutHeterozygous MiceTransgenic MiceWildtype ControlEar Notching

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