Performing 1D Thin Layer Chromatography

Source: Laboratory of Dr. Yuri Bolshan — University of Ontario Institute of Technology

Thin layer chromatography (TLC) is a chromatographic method used to separate mixtures of non-volatile compounds. A TLC plate consists of a thin layer of adsorbent material (the stationary phase) fixed to an appr...

Thin layer chromatography, or TLC, is a chromatographic method used to separate mixtures of non-volatile compounds, commonly used in organic chemistry.

TLC is performed on a glass- or plastic-backed plate. A baseline is marked on the plate, along with labels. The mixture being examined and reference compounds are dissolved in an appropriat...