Bioreactor Design and Operational System

A bioreactor is a vessel used to culture microorganisms under controlled conditions to produce a biological product while minimizing contamination of the culture and the final product.

The most commonly used industrial bioreactor is the aerated stirred-tank batch bioreactor.

It is typically made up of stainless steel. Also, it includes a transfer line and an inoculum port for introducing cultures, as well as sight and light glasses for observation.

The charge hole is used to add materials, such as buffers, nutrients, or antibiotics, while steam and water jacket help regulate temperature. Additionally, an air exhaust is present to release gases.

Other components of the bioreactor are baffles for enhanced mixing and an impeller, which aids in uniform stirring. A sparger plate introduces air or gas to help aeration, while an outlet at the bottom allows the product to be drained or harvested.

To prevent contamination, bioreactors are fully welded, with smooth internal surfaces and non-porous gaskets at all joints.

Bioreactors are also equipped with sensors to monitor pH, temperature, and dissolved oxygen, ensuring optimal productivity.