Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart

Abstract

Source: Li, Y. et al. Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice. J. Vis. Exp. (2021).

In this video, we demonstrate the procedure to surgically remove the left ventricular apex of a neonatal mouse heart.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Apical resection operation in neonatal 1-day-old Cx3cr1 ^GFP/+ mouse (C57BL/6 background)

1. Anesthesia
   1. Take all the mouse pups out from the cages and put them in a clean dry box.
   2. Embed each mouse in the ice for about 2-3 minutes. Ensure that there is a thin barrier such as latex or gauze between the ice and the mouse pup to prevent frostbite. 
   3. Identify ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Neonatal Heart Dissociation Kit	Miltenyi Bio Tech, Teterow, Germany	130-098-373