Establishing a Mouse Model of Small Fiber Neuropathy Using Resiniferatoxin

Abstract

Source: Lee, Y. et al., Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1. J. Vis. Exp. (2018).

This video demonstrates a method for establishing a small fiber neuropathy model in mice using the TRPV1 agonist resiniferatoxin (RTX). It outlines the steps involved in administering RTX to mice and explains how RTX induces neuronal death in small-diameter sensory neurons, thereby establishing the neuropathy model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Establishment of Resiniferatoxin (RTX) Neuropathy

CAUTION: RTX is neurotoxic and hazardous. On contact, it acts as an irritant to the eyes, mucous membranes, and upper respiratory tract. Avoid inhalation and wear lab eyeglasses and coats during RTX preparation. Rinse with plenty of water in case of skin contact or after handling.

1. Add 1 mg of RTX powder to a 200 µL mixture of equal volume of Tween 80 and absolute ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Chemical reagent
Resiniferatoxin	Sigma	R8756
Tween 80	Sigma	P1754