Establishing an Alzheimer's Disease Rat Model Through an Amyloid-Beta Peptide Injection

Abstract

Source: Xiaoguang, W., et al. Establishment of a Valuable Mimic of Alzheimer's Disease in Rat Animal Model by Intracerebroventricular Injection of Composited Amyloid Beta Protein. J. Vis. Exp. (2018).

This video demonstrates the procedure for injecting amyloid-beta peptides, aluminum chloride, and a neuroinflammatory growth factor, which collectively aids in toxic amyloid plaque formation in the hippocampus region. This causes memory impairment in the rat, characteristic of Alzheimer's disease.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgical Procedure

Note: 20 male Sprague-Dawley rats were microinjected with composited amyloid beta protein (Aβ) into the lateral ventricle and anterodorsal thalamic nucleus and designated as the composited Aβ-treated group. Another 20 rats were subjected to the same operation but received 0.1% dimethyl sulfoxide (DMSO) saline microinjection and were designated as the sham-operated group.

1. Anesthetize the rats wi....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Sprague-Dawley rat	Beijing Vital River Laboratory Animal Technology Co., Ltd, China	SCXK(Jing) 2012-0001	300–350 g
Movable small animal anesthesia	RWD Life Science Co., Ltd. China	R580
Brain stereotaxic apparatus	RWD Life Science Co., Ltd. China	68001
Flexible bone drill	Shanghai Soft Long Technology Development Co., Ltd. China	BW-sD908
Transmission electron microscope	Japan Co., Ltd. Japan	JEM-1400
Two channel microinjection pump	RWD Life Science Co., Ltd. China	RWD202
EM microtome	Hitachi Co., Ltd. China	H-7650
Dummy cannula	RWD Life Science Co., Ltd. China	62001	0.D.0.64×I.D.0.0.45mm/M3.5 http://www.rwdls.com/English/Product/3985102014.html
Guide cannula	RWD Life Science Co., Ltd. China	62101	0.D.0.40mm/M3.5
Internal cannula	RWD Life Science Co., Ltd. China	62201	0.D.0.41×I.D.0.25mm/M3.5
Tighten the nut	RWD Life Science Co., Ltd. China	62501	0.D.5.5mm/L7.5mm/M3.5
Fixing screw	RWD Life Science Co., Ltd. China	62514	M1.2×L2.0mm(100BAO)
The screwdriver	RWD Life Science Co., Ltd. China	62999	45*1mm
PE Tubing	RWD Life Science Co., Ltd. China	62302
Amyloid beta 25-35	Sigma Aldrich Co. USA	SCP0002-5MG
Recombinant human transforming growth factor-β1	PeproTech Inc. USA	100-21
Aluminium trichloride	Tianjin Kemiou Chemical Reagent Co., Ltd. China	3011080
Zinc phosphate dental cement	Dental Material of Factory Shanghai Medical Instruments Co., Ltd. China	201311