Demyelination and Remyelination of Mouse Spinal Cord Neurons

In This Article

Abstract

Source: Keough, M. B., et al. Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin. J. Vis. Exp. (2015)

This video demonstrates the method for inducing demyelination in mouse spinal cord neurons using lysolecithin injections, followed by remyelination through the migration and differentiation of oligodendrocyte precursor cells. The process impairs and then gradually restores neuronal signal transmission.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Prepare Syringe for Injection

Dissolve lysolecithin in a 1% solution in phosphate-buffered saline (PBS; pH 7.4) and store at -20 °C in small aliquots (75 μl). Thaw a vial to room temperature (RT).
NOTE: If lysolecithin is undissolved, sonicate the tube in an ultrasonic cleaner (40 kHz) for approximately 30 min to form a uniform solution.
Handle the pre-pulled glass capillary with extreme care to avoid damaging the delicate ....

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Results

Diagram of microinjection setup; syringe components, priming, and lysolecithin injection process.
Figure 1. Assembly of the injecting syringe. (A) The nut of the injecting syringe is threaded onto the flat end of the glass capillary, followed by the two ferrules such that their mating ends interlock. Once the capillary is firmly snug in the conical fer.......