Symmetric Bihemispheric Sectioning of the Human Postmortem Brain

In This Article

Abstract

Source: Iacono, D., et.al., Symmetric Bihemispheric Postmortem Brain Cutting to Study Healthy and Pathological Brain Conditions in Humans. J. Vis. Exp. (2016)

The video demonstrates a step-by-step procedure for symmetrically dissecting a fixed postmortem human brain, preparing smaller tissue blocks for further analysis.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

NOTE: The protocol describes a symmetric bihemispheric brain cutting procedure for postmortem brain assessment finalized for neuropathological studies in humans. Detailed descriptions of the apparatuses, instruments, materials, and supplies necessary for human brain cutting will be excluded. Materials and supplies for brain dissections are selected at the discretion of the single investigator and are based on autopsy tools allowed or approved at each research institution. The minimal set of tools and materials r....

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Results

Brain dissection process, labeled anatomy, cerebrum, cerebellum, pre-central and post-central gyrus diagram.
Figure 1. Preliminary Brain Assessment Before Bihemispheric Brain Cutting. This figure shows the various superficial aspects of a human brain after 2 weeks of fixation in 10% neutral-buffered formalin. In a clockwise sequence, t.......