Time-Lapse Imaging of Cortical Neuron Radial Migration in Transduced Mouse Embryonic Brain Slices

Abstract

Source: Wiegreffe, C., et. al., Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation. J. Vis. Exp. (2017)

This video demonstrates time-lapse confocal microscopy to observe cortical neuron radial migration from the subventricular zone to the cortical plate via radial glial scaffolds in transduced mouse embryonic brain slices.

Protocol

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
6-well plate	Corning	351146
Inverted fluorescence tissue culture microscope	Leica	DM IL LED
Confocal laser scanning microscope	Leica	TCS SP5II
Hybrid detector	Leica	HyD
Objective, 40x/0.60 NA	Leica	11506201
Microscope temperature control system	Life Imaging Services	Cube, Brick & Box
Cell culture insert	Millipore	PICM0RG50
Microgrinder	Narishige	EG-45	Use 38° angle for beveling
Microinjector	Parker Hannifin	052-0500-900	Picospritzer III
Tweezers with 5 mm platinum disk electrodes	Sonidel	CUY650P5
Micropipette puller	Sutter Instrument	P-97
Box filament	Sutter Instrument	FB255B	2.5 mm x 2.5 mm
Micro-spoon spatula	VWR	231-0191	185 mm x 5 mm
Glass bottom dish, 50 mm	World Precision Instruments	FD5040-100