Contrast-Enhanced Micro-CT Imaging of Cerebral Cavernous Malformation Lesions in a Mouse Brain

Abstract

Source: Choi, J. P. et al. Induction and Micro-CT Imaging of Cerebral Cavernous Malformations in Mouse Model. J. Vis. Exp. (2017)

This video demonstrates the use of micro-CT imaging to visualize cerebral cavernous malformation (CCM) lesions in a mouse brain. It details the steps for sample preparation, imaging, and 3D image reconstruction to evaluate the density and location of CCM lesions in brain tissue.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

Results

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
4-hydroxy tamoxifen	Sigma-Aldrich	H6278	To activate Cdh5-CreErt2
Corn oil	Sigma-Aldrich	C8267-500ML	To dilute 4-hydroxy tamoxifen
Stereomicroscope	Leica	M205FA	To take macroscopic images
Lugol's Iodine solution	Sigma-Aldrich	L6146	To stain samples for contrast micro-CT
Plastic paraffin film	Parafilm	PM992	To package samples
Micro-CT	Xradia	MicroXCT-400	Micro-CT
3D rendering software	FEI Visualization Science group	Avizo 3D image processing software	To analyse micro-CT scans