A Sensitive Visual Method for the Detection of Hydrogen Sulfide-Producing Bacteria

In This Article

Abstract

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

Source: Zhu, W., & Chu, W. Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria. J. Vis. Exp. (2022)

This video demonstrates a sensitive visual method for the rapid detection of hydrogen sulfide–producing bacteria. A color change from yellow to black indicates bacterial activity through the formation of bismuth sulfide.

Protocol

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

1. Bacterial strains

NOTE: For this experiment, nine standard strains were used, including Salmonella paratyphi A, Salmonella paratyphi B, Fusobacterium nucleatum, Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa PAO1, Aeromonas hydrophila YJ-1, Proteus vuigaris, and Klebsiella pneumoniae. Salmonella paratyphi A, Fusobacterium nucleatum, Pseudomonas aeruginosa, and Proteus vuigaris can produce H₂S.

Preparation of bacterial culture
1. Transfer one bacterial colon....

Access restricted. Please log in or start a trial to view this content.

Materials

List of materials used in this article

Name

Company

Catalog Number

Comments

Bismuth (III) chloride

Shanghai Macklin Biochemical Co., Ltd

7787-60-2

EDTA

Nanjing Chemical Reagent Co., Ltd

60-00-4

Fusobacterium nucleatum

ATCC

25586

L-cysteine

Amresco

52-90-4

Triethanolamine-HCl

Shanghai Aladdin Biochemical Technology Co., Ltd.

637-39-8

Sodium hydrosulfide

Pipette

Reprints and Permissions

Request permission to reuse the text or figures of this JoVE article

Request Permission