In this report, we propose a new application of light-curing dental resins for mounting base of electrodes or microdialysis probes in chronic experiments. This material allows direct bonding to the cranium.
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Method Article
Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
In This Article
Summary
Abstract
In chronic recording experiments, self-curing dental acrylic resins have been used as a mounting base of electrodes or microdialysis-probes. Since these acrylics do not bond to the bone, screws have been used as anchors. However, in small experimental animals like finches or mouse, their craniums are very fragile and can not successfully hold the anchors. In this report, we propose a new application of light-curing dental resins for mounting base of electrodes or microdialysis probes in chronic experiments. This material allows direct bonding to the cranium. Therefore, anchor screws are not required and surgical field can be reduced considerably. Past experiences show that the bonding effect maintains more than 2 months. Conventional resin's window of time when the materials are pliable and workable is a few minutes. However, the window of working time for these dental adhesives is significantly wider and adjustable.
Protocol
Step 1. Preparation of probe implantation
- Anesthesia (not shown in the video)
Anesthetize each bird with an interperitoneal injection of a ketamine (12.5 mg/kg body weight) and xylazine (25 mg/kg) mixture to produce a steady level of surgical anesthesia. - Fix a head to a stereotaxic frame
Use Xylocaine(TM) jelly to reduce local pains of ear bars' pressure. The head of bird is fixed in a stereotaxic apparatus consisting of ear bars and a beak holder that holds the head at a 45° angle. During the stereotaxic surgery for implantation, warm the body of bird by surgical pad with thermal contro....
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Discussion
Compared to the application of conventional self curing acrylic resins, the introduced technique has several important advantages:
1. Direct bonding:
Self curing acrylic can be attached to the bone for a while but it does not adhere to the bone. Therefore, anchorage screws are required to fix a mounting base (See Figure 2A). In contrast, light curing resin can bond to the bone directly so screws are not required (Figure 2B). Therefore, size of mounting base can be reduced considerab.......
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Disclosures
Experiments were undertaken in accordance with the guidelines for animal experimentation of the brain science institute, RIKEN (RIKEN-BSI) and the institute's animal ethics committee approved them.
Acknowledgements
We thank our colleagues in RIKEN-BSI for their kindly supports and fruitful advices during this work. I would like to express my sincere appreciation to Professor Shigemi Mori, Professor G bor Juh sz, Drs. Kiyoji Matsuyama, Katalin A K kesi and rp d Dobolyi who advised me to study microdialysis method during the graduate course at the Graduate University for Advanced Studies, Japan. Finally, we thank Mr. Chris Sarnecky and Dr. Akitsugu Takasu for their supports in video recording.
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Materials
| Name | Company | Catalog Number | Comments | |
|---|---|---|---|---|
| Bengalese finches | Animal | Two adult male , Body weight: 14.2 g and 14.8 g | ||
| Microdialysis probe | Tool | EICOM | total weight of probe, guide cannula, cap nut and dental adhesive resin: 0.65 to 0.78 g. | |
| Light-cured dental adhesive resin system | Kuraray Medical Inc | +Light cured composite resin (CLEARFIL AP-X). +Dual-cure dental adhesive system with the self-etching primer and bonding agent (CLEARFIL Liner Bond IIΣ). | ||
| Surgical instruments | Stereotaxic frame, surgical microscope, thermal controlled surgical pad and thermal controlled recovery chamber. Plain plugger for handling composite resin. Microspatula or sharp curette to ablate periosteum and muscles.Injection syringe and needle (30G) for inducing anesthesia.Small scissors. Fine forceps for making small craniotomy. | |||
| Light-unit | Rolence Enterprise Inc | Ultra-Lite 500E | ||
| Microscope | Konan Medical | KOM300 | with C-mount adaptor | |
| 3- CCD camera | Tool | Hitachi | HV-D30 |
References
- Adell, A., Artigas, F. In vivo Brain microdialysis: Principles and applications. In Vivo Neuromethods: Neuromethods. Boulton, A., Baker, G., Bateson, A. 32, Humana Press Inc. Totowa. 325-357 (1998).
- Kendrick, K. Microdialysis in large unrestrained animals: neuroendocrine and behavioral studies of acetylcholine, amino acid, monoamine and neuropeptide release in the sheep.
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