Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS)

Abstract

Source: Xue, P., et al. A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome. J. Vis. Exp. (2018).

This article describes a way to prepare and insert dihydrotestosterone (DHT) pellets to model Polycystic Ovary Syndrome (PCOS) in mice. The example protocol demonstrates the procedure in mice used for reproductive and metabolic studies.   

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Create PCOS-like Mouse Model

NOTE: The mice used in this study were a mixed background (C57/B6, CD1, 129Sv) and were maintained with food and water ad libitum in a 14/10 h light/dark cycle at 24 °C.

1. DHT Pellet Preparation
   1. Autoclave silastic tubing to sterilize. Cut silastic tubes to a length of 15 mm with a razor blade.
   2. Seal one side by injecting medical adhes....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Crystalline 5α-DHT powder	Sigma-Aldrich	A8380-1G
Dow Corning Silastic tubing	Fisher Scientific	11-189-15D	0.04in/1mm inner diameter x 0.085in/2.15mm outer diameter
Medical adhesive silicone	Factor II, InC.	A-100
Goggles, lab coats, gloves and masks
3 mL Syring	Becton, Dickinson and Company (BD)	30985
Attached needle: 20G	BD	305176
Ruler: any length than 10 cm with milimeter scale.
Xylazine	Vet one AnnSeA LA, MWI, Boise	NDC13985-704-10	100 mg/mL
Ketamine Hydrochloride	Hospira, Inc	NDC 0409-2051-05	100 mg/mL
Surgical staple	AutoClip® System, Fine Science Tool	12020-00
Insulin syringe	BD	329461	1/2 CC, low dose U-100 insulin syringe
Trocar	Innovative Research of America	MP-182
Razor blade	Fisher Scientific	12-640
Clidox	Fisher Scientific	NC0089321
Surgical underpad	Fisher Scientific	50587953	Andwin Scientific 56616018
Betadine Antiseptic Solution	Walgreens
3M Vetbond (n-butyl cyanoacrylate)	3M Science. Applied to Life