Systemic Pilocarpine Treatment: Mouse Model of Temporal Lobe Epilepsy

Abstract

Source: Kim, J. E. and Cho, K. O. The Pilocarpine Model of Temporal Lobe Epilepsy and EEG Monitoring Using Radiotelemetry System in Mice. J. Vis. Exp. (2018).

This video presents the procedure for inducing status epilepticus (SE) in mice by systemic administration of pilocarpine to model temporal lobe epilepsy.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. SE Induction

1. Purchase 8-week-old male C57BL/6NHsd mice and weigh each mouse. Then, use a marker pen to mark the tails of all mice for their easy identification during SE induction.
2. Calculate the amount of scopolamine methyl bromide (scopolamine; 2 mg/kg), terbutaline hemisulfate (terbutaline; 2 mg/kg), and pilocarpine hydrochloride (pilocarpine; 280 mg/kg) based on the mouse weight and add saline (0.9% NaCl, 10 mL/kg) to make solutions.
   NOTE:....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
C57BL/6	Envigo	C57BL/6NHsd
Scopolamine methyl nitrate	Sigma	S2250	Make 10X stock
Terbutaline hemisulfate salt	Sigma	T2528	Make 10X stock
Pilocarpine hydrochloride	Sigma	P6503
Diazepam	SAMJIN
Castor oil (Kolliphor EL)	Sigma	C5135	Polyoxyl 35 hydrogenated castor oil
Saline	Daihan pharm. Co.
5% Dextrose	Daihan pharm. Co.
1 mL syringe	Sung shim medial Co.  Ltd.
26 guage needle	Sung shim medial Co.  Ltd.		26 G * 13 mm (1/2")
30 guage needle	Sung shim medial Co.  Ltd.		30 G * 13 mm (1/2")