Antibody-Functionalized SERRS Nanoprobe Based Imaging: A Highly Sensitive Raman-imaging Technique to Detect Metastatic Ovarian Cancer In Vivo

Abstract

Source: Andreou, C. et al. Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting. J. Vis. Exp. (2019)

This video describes the surface-enhanced resonance Raman scattering technique to detect metastatic ovarian cancer using targeted nanoprobes specific anti-receptor antibodies and non-targeted control nanoprobes. This method helps to determine the relative abundance of the two nanoprobes and differentiate the cancer cells from the visceral background.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Gold Nanostar Core Synthesis

NOTE: Gold nanostars are used as cores for both flavors of SERRS nanoprobes used in this experiment.

1. Prepare 800 mL of 60 mM ascorbic acid (C₆H₈O₆) solution in deionized (DI) water and 8 mL of 20 mM tetrachloroauric acid (HAuCl₄) solution in DI water. Cool to 4 °C.
2. Perform this reaction step at 4 °C. Place a conical flask containin....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Name of Reagent
Ascorbic acid	Sigma-Aldrich	A5960
3-MPTMS	Sigma-Aldrich	175617
Ammonium hydroxide (28%)	Sigma-Aldrich	338818
Anti-Folate Receptor antibody [LK26]	AbCam	ab3361
Dimethyl sulfoxide	Sigma-Aldrich	276855
Dimethyl sulfoxide (anhydrous)	Sigma-Aldrich	276855
Ethanol	Sigma-Aldrich	792780
IR140	Sigma-Aldrich	260932
IR780 perchlorate*	Sigma-Aldrich	576409	Discontinued*
Isopropanol	Sigma-Aldrich	650447
N.N.Dimethylformamide	Sigma-Aldrich	227056
*IR792	Sigma-Aldrich	425982	*Alternative
Tetraethyl Orthosilicate	Sigma-Aldrich	86578
PEG-maleimide	Sigma-Aldrich	900339
PEG crosslinker	Sigma-Aldrich	757853
Tetrachloroauric Acid	Sigma-Aldrich	244597
Name of Equipment
Dialysis cassette (3,500 MWCO)	ThermoFisher	87724
Centrifugal filters	Millipore	UFC510096
inVia confocal Raman microscope	Renishaw