Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model

Abstract

Source: Krishnan, V. et al. Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose. J. Vis. Exp. (2015)

In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent metastasis. 

Protocol

All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Intraperitoneal Injection of Cells

NOTE: In our experiments, mice are handled in a laminar airflow or biosafety cabinet within our barrier facility in order to limit the risk of pathogen exposure. In order to demonstrate this technique clearly, the procedures in the accompanying video were conducted in a laboratory approved for animal work. This particular technique does not require the animals to be under anesthesia. Under approved prot....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
DMEM	Corning	10-013-CV
PBS	Corning	21-040-CV	Without calcium and Magnesium
26 gauge needle	BD	329652