Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model

Abstract

Source: Sheets, K. T. et al. Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds. J. Vis. Exp. (2018)

This video presents the surgical procedure for image-guided tumor resection and implantation of a bio-scaffold seeded with genetically engineered mesenchymal stem cells or MSCs expressing tumor necrosis factor-related apoptosis-inducing ligand or TRAIL. These therapeutic MSCs have the potential in treatment of brain tumors.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Fluorescence-guided Resection and Scaffold Implantation

NOTE: Sterilize all tools prior to initializing surgery.

1. Place the stereotaxic frame on the stage of a fluorescence dissecting stereomicroscope.
2. Anesthetize the mouse under inhaled isoflurane in an induction chamber. Once anesthetized, secure the mouse in the stereotaxic frame with continuous inhaled anesthesia supply via an isoflurane nose cone adapter. Mai....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Just for mouse stereotaxic instrument	Stoelting	51730	Maintains steady head positioning during surgery
Fluorescence dissecting stereomicroscope	Leica	M165 FC	Allows real-time imaging of tumor during resection
Vetbond tissue adhesive	3M	1469	Sugical glue to close skin wound
Artificial tears	Akorn	664268	Prevents eyes from drying during surgery
Webcol alcohol preps	Covidien	6818	Sterilize incision site
Betadine surgical scrub	Purdue Fredick Company	6761815117	Sterilize incision site
Cotton-tipped applicators	Fisherbrand	23-400-115	Surgery tool
E-vac aspirating system	Argos	EV310	Vacuum pump used to resect tumor
Fibrinogen and thrombin extracted from as-received TISSEEL	Baxter		To temporarily secure the scaffold in the resection cavity