Murine Perineural Invasion Model: Generating an In Vivo Model to Simulate Perineural Invasion in Mouse Sciatic Nerve

Abstract

Source: Deborde, S. et al. An In Vivo Murine Sciatic Nerve Model of Perineural Invasion. J. Vis. Exp. (2018)

In this video, we develop an in vivo mouse model for perineural invasion by injecting pancreatic cancer cells into the sciatic nerve. This model helps quantify the extent of invasion and study the interactions between cancer cells and nerves.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of the Mice and Surgery

NOTE: 8-week-old, male and female C57BL/6J mice are used in this study. The surgery conditions follow the IACUC rules of our institution. The instruments are sterilized, the surgical working surface is disinfected, the animal is disinfected, and the surgeon wears sterile gloves.

1. On the day before the surgery, anesthetize the mouse using 2% isoflurane and then remove the fur along the length of femur on the dorsal side wit....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Mouse			Number and age variable depending on experimental needs
Electric razor	WAHL	9962	Can be substituted with commercial hair removal agent
Isoflurane, 250 mL	Baxter	1001936060
Hypoallergenic surgical tape	3M Blenderm	70200419342
Betadine Swapsticks	PDI	SKU 41350
Webcol Alcohol Preps	Covidien	5110
Sterile surgical tools (scissors and forceps)
10 μL Hamilton syringe	Hamilton	80308
Steel Micro spatula	Fisher Scientific	S50823
Dissecting microscope
Bupivacine, 1 g	Enzo Life Sciences	BML-NA139-0001	Reconstitute to 0.5%
5-0 Nylon suture	Ethicon	698H