Catheter Based Intravenous Injection of Experimental Agents: A Technique for Delivering Experimental Agents Intravenously Through Jugular Vein in Mouse Model

Abstract

Source: Bornert, A. et al. In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow. J. Vis. Exp. (2021)

In this video, we describe a method for implanting a catheter into the jugular vein of a mouse model through which experimental agents such as drugs and tracer dyes can be delivered intravenously in vivo. Such an arrangement when connected to other systems can also be used for monitoring hemodynamics and blood sampling.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of mice and insertion of a catheter in the jugular vein

NOTE: Here, male or female, 5-7-week-old mTmG reporter mice were used (B6.129(Cg) Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo10) crossed with Pf4-cre mice, allowing intense green fluorescence labeling in megakaryocytes and platelets. Before beginning the experiment, preheat the heating chamber of the microscope for a few hours.

1. Transport the mouse to ....

Results

Figure 1: Insertion of the catheter. (A) An incision is made to expose the jugular vein and the top of the pectoral muscle.

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Histoacryl 5 x 0, 5 mL 	Braun 	1050052	Injectable solution of surgical glue
Imalgene/Ketamine 1000 fl/10 mL 	Boehring 	3661103003199.00	Eye protection
Rompun Xylazine 2% fl/25 mL	 Bayer 	4007221032311.00
Surflo IV catheter - Blue 22 G 	Terumo 	SR-OX2225C1