Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye

Abstract

Source: Okurowska, K., et al. Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis. J. Vis. Exp. (2020).

In this video, we show a procedure to surgically isolate the corneoscleral button to generate an ex vivo porcine cornea model. The model is helpful for histological, pathological, and pharmacological studies due to its similarity to the human eye.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Sterilization

1. CRITICAL STEP: Disinfect all forceps and scissors by soaking for 1 h in 5% (v/v) solution of Distel in distilled water, clean with a brush, rinse with tap water and sterilize in an oven at 185 °C for a minimum of 2 h.
2. Sterilize all other glassware and reagents by autoclaving at 121 °C for 15 minutes or prepare reagents according to the manufacturer's instructions. Carry out the following procedures in a class II microbiology safety c....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Cellstar 12 well plate	Greiner Bio-One	665180
Distel	Fisher Scientific	12899357
Forceps	Fisher Scientific	15307805
PBS	SLS	P4417
Petri dish	Fisher Scientific	12664785
Povidone iodine	Weldricks pharmacy	2122828
Scalpel blade number 15	Fisher Scientific	O305
Scalpel Swann Morton	Fisher Scientific	11849002