Intrathecal Placement of Lumbar Drain: A Technique to Withdraw Cerebrospinal Fluid from the Lumbar Spine in Porcine Model

Abstract

Source: Behem, C. R. et al., Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion. J. Vis. Exp. (2020).

This video describes the technique of intrathecal placement of a lumbar drain in a porcine ischemic model, which helps in draining the cerebrospinal fluid to a drainage system. The cerebrospinal fluid pressure is monitored by the drainage system, and fluid is drained to maintain a constant cerebrospinal fluid pressure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

The study was performed in six three-month-old male and female pigs (German Landrace) weighing approximately 40 kg. Animals were brought to the animal care facilities at least 7 days prior to the experiments and were housed in accordance to animal welfare recommendations. Animals were provided food and water ad libitum, and their health status was regularly assessed by the responsible veterinarian. A fasting time of 12 h was maintained prior to the experiments. The entire experimental procedure and....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
LiquoGuard 7	Möller Medical GmbH, Fulda, Germany		Cerebrospinal Fluid Drainage System
Twinwarm	Moeck & Moeck GmbH, Hamburg, Germany	12TW921DE	Warming System
Universal II Warming Blanket	Moeck & Moeck GmbH, Hamburg, Germany	906	Warming Blanket
Zeus Dräger Medical,	Lübeck, Germany		Anesthesia Machine